Centrifuge-based micromixer with three-dimensional square-wave microchannel for blood plasma mixingPDMSProthrombin TimeCompact DiskInlet ChannelMicrofluidic PlatformA numerical and experimental investigation is performed into the flow characteristics and mixing performance of four centrifuge-based compact disk (CD...
Introduction Protocol Representative Results Discussion Acknowledgements Materials References English Explore More Videos Keywords 3D Tumor Spheroid Invasion Assay Cancer Glioblastoma Squamous Cell Carcinoma EGFR Matrix Invasion Drug Screening Image Analysis
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After thawing tissue overnight at 4°C, tissue was minced into pieces ~1 cm3 in size. Minced tissue was rinsed with sterile water (Baxter, Deerfield, USA) to remove excess blood and fluids. Next, tissue was blended in sterile water (blender model 7010S, Waring Commercial, Torrington, USA...
Staining efficiency of our technique is improved over conventional staining because of the flow rate in 3D-μDialysis staining. Moreover, the staining process has been validated with clinical whole blood samples from three TNM stage IV colon cancer patients. The current technique may be termed as ...
Stem cells therapy offers a viable alternative for treatment of bone disorders to the conventional bone grafting. However clinical therapies are still hindered by the insufficient knowledge on the conditions that maximize stem cells differentiation. Here
Centrifuge machine Standard design The machine consists of a frame that has a horizontal drive shaft with clutch and brake, worm gear , lubricating oil bath and vertical bowl spindle in the lower part. The bowl is mounted on top of the spindle in the...
A representative tissue upon resection of engineered tissues at day 7 demonstrates adhesion to host tissue and the presence of bloodcarrying vessels. Ter-119 positive red blood cells (green) were identified in these explants near but not typically immediately juxtaposed to arginase-1 (ARG-1, red)...
is synthesized and secreted from liver cells. Its main function is to regulate the colloidal osmotic pressure of blood36. CYP1A1 enzyme catalyzes the oxygenation of polycyclic aromatic hydrocarbons (PAHs) and heterocyclic aromatic amines/amides (HAAs), resulting in the formation of chemical carcinogens...
Hemolysis was measured on human red blood cells (taken from healthy volunteers). Briefly, a suspension of human erythrocytes was mixed with two-fold serial dilutions of each tripeptide and this mixture was then incubated for 1 h at 37 °C. Subsequently, the samples were centrifuged for 5 min...