Both forms migrated on an SDS-PAGE gel at the same position as the Mpro-NE monomer, with or without DTT, suggesting that the formation of D-Mpro-NE is not through a disulfide bond, even though Mpro-NE has 12 free cysteine residues. Similar to the N-finger deletion mutant Mpro-∆...
The assay measures production of free CoA, which can be produced either by proper condensation or futile hydrolysis of the thioester bond of acyl-CoA substrates (Fig. 1). X. campestris OleA has been previously shown to prefer C16 and C14 acyl-CoA substrates (6). The C14 acyl-CoA substrate...
2, 3 According to the disulfide-bond pattern of β3 proposed by Calvete et al,4 residue 33 is located within a thirteen amino acid loop formed by Cys26-Cys38. The search for the precise epitopes recognized by alloantibodies that distinguish this polymorphism has been complicated by a number...
However, a very recent example from the Silverman's group showed that DNA featuring protein-like functional groups could be selected for the hydrolysis of an amide bond inserted within an oligonucleotide as substrate [4]. Inspired by the composition of the active site of serine-protease in which...
bond_ipv6.c: 5432 bond_main.c: 137243 bond_sysfs.c: 42050 bonito64.h: 16247 bonito-irq.c: 2465 booke.c: 16034 booke_emulate.c: 6991 booke.h: 2429 booke_interrupts.S: 11644 booke_wdt.c: 4745 boot: 4096 boot2.H16: 14566 boot.c: 8143 bootcode.bin.ihex: 604...