To maintain selection pressure, add 10 µg/ml Blasticidin, 1 µg/ml Puromycin, and 100 µg/ml Zeocin® to the growth medium every other passage. 3. Pass the cells every 3 days by inoculating 5 x 105 cells/ml. Do not allow the cell concentration to exceed 2 x 106 cells/...
First of all, we should ensure the THP-1 cells are successfully infected with virus. For this purpose, we could choose the retroviral vector with fluorescent proteins (GFP or RFP). Then, ensure that the concentration of puromycin is not too high. The appropriate concentrations of puromycin shou...
1.2.3puromycin 筛选稳定株 1.2.2 72h LV5-SP110 LV5-NC 1)按照 中掌握的实验条件感染靶细胞,感染 后 与 组分 5 puromycin 3.5 4 4.5 5 10ug/ml 别设 孔,将 稀释至 、、、 的浓度,再将不同浓度的抗 生素分别加入每组,观察并记录48h后细胞全部死亡对应的浓度。 6 1 2)选用 孔板用步骤 )确定的抗...
International Journal of Molecular Sciences Article 5-(3 ,4 -Dihydroxyphenyl-γ-valerolactone), a Major Microbial Metabolite of Proanthocyanidin, Attenuates THP-1 Monocyte-Endothelial Adhesion Charles C. Lee 1,† , Jong Hun Kim 2,† , Ji Seung Kim 3 , Yun Sil Oh 3 , Seung Min Han 3,...