Dual Reporter THP-1 Cells with stable overexpression of TLR7 ± a knockout of TLR8. These cells allow the simultaneous study of NF-kB and IRF pathways.
THP1-Dual™ KO-RIG-I Cells Human THP-1 Monocytes - RIG-I knockout NF-κB-SEAP and IRF-Lucia Reporter Cells Add to favorite 3-7 x 10e6 cells thpd-korigi ￥26,704 Please contact our distributor THP1-Dual™ KO-RIG-I vial Additional cell vial Add to favorite 3-7 x ...
图3.2 Reporter方法检测 2’3’-cGAMP对 THP1-Dual细胞的抑制作用 3.2.2 THP1-STING-KO 细胞系 爱思益普细胞学团队同时构建了THP1-STING-KO 细胞系检测STING蛋白表达水平(图3.3)。构建的细胞系具有调节细胞系中不同基因的水平(图3.4)。 图3.3 THP1-STING-KO细胞系中STING蛋白表达水平 图3.4 THP1-STING-KO...
THP1-Dual™ Cells THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs. As a result, THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF ...
The results of dual luciferase reporter gene assay showed that miR-126-3p bound to the 3'UTR of RGS3. Western blotting assay showed that the RGS3 protein expression level in THP-1 cells of the miR-126-3p overexpression group was lower than that in the control group. ConclusionsmiR-126-...
4.1.1 THP1-XBlue™ THP1-XBlue™ is derived from the THP-1 cell line via transfection with a reporter construct expressing a secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter that is inducible by the transcription factor NF-κB and AP-1. If such transcrip...
When SHP was over-expressed in foam cells, SCD1 expression was significantly reduced (Figure 2F). Figure 2 Walnut oil inhibits SCD1 through a FXR pathway in THP-1 MDFC. (A) Activation of walnut oil on nuclear receptor ligand binding domain of Dual Luciferase Reporter Assay. PPAR: ...
The transfected cells were cultured for 48 h, and the luciferase activities of DC-SIGN promoter luciferase reporter plasmids and the inner control pRL-TK were detected using the dual luciferase reporter assay kit (Promega) in GloMax96 microplate luminometer (Promega). The relative activity of ...
The results of dual luciferase reporter gene assay showed that miR-126-3p bound to the 3'UTR of RGS3. Western blotting assay showed that the RGS3 protein expression level in THP-1 cells of the miR-126-3p overexpression group was lower than that in the control group. ConclusionsmiR-126-...
THP1-Dual cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs. THP1-Dual cells allow the simultaneous study of the NF-kB pathway, by monitoring the activity of SEAP, and the IRF pathway, b