A general kinase reaction was developed based on the differential reactivity of ATPbetaS and ADPbetaS torward bis-dithio nitrobenzoic acid (DTNB). ADPbetaS, an analog of ADP, the common product of all kinase (or ATPase) reactions is detected after undergoing a disulfide exchange reaction with ...
Catalog #: 92096-92099View all CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes. Quick Add Detailed View Product CF® Dye Maleimides From:$211Sizes:0.5 umol, 0.25 umol, 1 umolCatalog #...
Click chemistryFluorescent probesImaging agentsProteinsThiolsMaleinimides are widely used to label proteins and to derivatize thiols. The so-called click reaction was shown to allow the efficient introduction of the maleinimido group into azido-modified fluorophores (benzoxazines) by reacting them with ...
关键词: Click chemistry Fluorescent probes Imaging agents Proteins Thiols DOI: 10.1002/ejoc.201001085 被引量: 10 年份: 2010 收藏 引用 批量引用 报错 分享 全部来源 求助全文 全文购买 Wiley Wiley Semantic Scholar 掌桥科研 dx.doi.org 查看更多 相似文献 参考文献 引证文献...
Introduction of paramagnetic probes into non-metal-binding proteins can be achieved through alkylation of unique cysteine residues with thiol-reactive EDTA (Fig. 1). As wild-type barnase lacks cysteine residues, a unique cysteine was introduced at position 102 using site-directed mutagenesis. The pur...
Thiol reactive probes: Applications in kinase assays and thiol detection. A general kinase reaction was developed based on the differential reactivity of ATPbetaS and ADPbetaS torward bis-dithio nitrobenzoic acid (DTNB). ADPbetaS... T Chiku - Marquette University. 被引量: 0发表: 2008年 ...
All fluorescent probes published to date either are incompatible with hydrophobic proteins/ligands, precluding analyses of transmembrane or membrane-associated proteins, or have excitation and detection wavelengths outside the range of real-time polymerase chain reaction (RT–PCR) machines, necessitating ...
All fluorescent probes published to date are either incompatible with hydrophobic proteins/ligands, which precludes analyses of transmembrane or membrane associated proteins, or have excitation and detection wavelengths outside the range of RT-PCR machines, necessitating the use of dedicated devices. ...
FLUORESCENT probesFLUORESCENCEProtein kinases are involved in signaling, making them favorable drug targets; hence the need to develop methods adaptable to high throughput assays and screening. We recently reported a UV Vis based kinase assay (Chiku et al, J Biomol Screen 2006 11: 844-853) ...
This makes thiol-reactive probes a favourite tool for protein modification. Modified bases like thiouridine also allow their use on nucleic acids. Excess reagent can be quenched with cysteine, DTT, or β -mercaptoethanol before it is separated from the labelled protein by dialysis or gel filtration...