Thiamine pyrophosphate (TPP), the biologically active form of thiamine (also known as vitamin B1), is an essential cofactor for several important enzymes involved in carbohydrate metabolism, and therefore, it is required for all living organisms. We recently found that a thiamine-binding protein (...
Thiamine pyrophosphate is the most active and abundant physiological state of thiamine in the body, responsible for over 80% of total body thiamine. AI generated definition based on: Vitamins and Minerals in Neurological Disorders, 2023 About this pageAdd to MendeleySet alert Also in subject areas...
8. 8. The reversibility of thiamine-binding activity was studied and it was found that the binding protein-thiamine complex was dissociated with difficulty in cold buffer for a period of several hours. 展开 关键词: Escherichia coli Carbon Isotopes Pyrimidines Thiazoles Thiamine Pyrophosphate ...
When necessary, 2 μM thiamine, thiamine monophosphate (TMP) and thiamine pyrophosphate (TPP), 1 μM hydroxymethylpyrimidine (HMP) and hydroxyethylthiazole (HET), 1 mg/mL root exudates and 5 mg/mL supernatant of thiamine-producing strain T-1 were added to the P1 medium for ...
The THI-box riboswitch, or how RNA binds thiamin pyrophosphate. Structure. 2007;15:259–65. Article CAS Google Scholar Terfehr D, Dahlmann TA, Specht T, Zadra I, Kurnsteiner H, Kuck U. Genome Sequence and Annotation of Acremonium chrysogenum, Producer of the beta-Lactam Antibiotic ...
Concentrations of thiamine pyrophosphate (b), thiamine (c) and thiamine monophosphate (d). Key: see Figure 3. Data are means ± SEM (n=6–13). * p<0.05 with respect to control study group; ** p<0.01 with respect to control study group; *** p<0.001 with respect to control study ...
described the 3D structure of the mouse thiamine pyrophosphokinase co-crystallized with AMP, pyrithiamine pyrophosphate (PPP) and magnesium ions [7]. Their results confirmed that the AMP (and maybe the ATP) is maintained in the protein by interactions with residues 57, 58, 77 to 79, 141 to...
Studies on the nature of the binding of thiamine pyrophosphate to enzymes The binding of thiamine pyrophosphate (TPP) to several enzymes has been determined by measuring cofactor-dependent activity after passage of each enzyme through a column of Sephadex G-25 to remove non-protein-bound cofactors....
mechanistic enzymology of thethiazoleand pyrimidine forming reactions, trapping of acarbocationintermediate at the active site of the thiazole/pyrimidine couplingenzymeand thiamin salvage. The use of protein thiocarboxylates in biosynthesis and a chemoenzymatic synthesis ofthiamin pyrophosphateare also ...
Subgroup 2.7.7 comprises a set of enzymes carrying out a nucleotidyl transfer and release of inorganic phosphate (with an NDP as substrate) or pyrophosphate (with an NTP as substrate): X- P- P(- P) + P- Y ⇔ X- P- P- Y + P(- P). Correspondingly, the AThTP-synthesizing ...