Thiamine-diphosphate-dependent (ThDP-dependent) enzymes are very efficient catalysts for asymmetric benzoin-type condensation. Through chemo- and stereoselective coupling of various carbonyl reagents promoted by enzymes of this family a wide variety of optically active α-hydroxy ketones have been produced...
The thiamine diphosphate dependent enzyme 2﹌eto acid decarboxylase (KdcA) from Lactococcus lactis is able to transfer formyl units to aliphatic and aromatic aldehydes, both in an electrophilic and in a nucleophilic way. We have established the formation of unstable chiral 2﹉ydroxy aldehydes by ...
The 3-deaza analogue of TPP (thiamine diphosphate), a close mimic of the ylid intermediate, has been synthesized and is an extremely potent inhibitor of a variety of TPP-dependent enzymes, binding much more tightly than TPP itself. Results using deazaTPP complexed with the E1 subunit of PDH ...
A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins Thiamine diphosphate dependent enzymes are well known for catalyzing the asymmetric synthesis of chiral -hydroxy ketones from simple prochiral substrates. ... R Westphal,C Vogel,C Schmitz,...
Thiamine diphosphate-dependent enzyme status due to thiamine deficiency in rat liverIt has been accepted that coenzymes have a stabilizing effect on the coenzyme-dependent enzymes beside their own functions as co enzyme (1-3). And also... Y Nose,K Takahashi,A Nakamura - 《Journal of Nutritiona...
Article: Molecular evolutionary analysis of the thiamine-diphosphate-dependent enzyme, transketolase
The thiazole biosynthetic enzyme of thiamine biosynthesis was highly expressed in the HY strain. Putative pyruvate oxidase and putative FAD-dependent oxidoreductase superfamily were involved in thiamine diphosphate (TPP) and FAD biosynthesis and were also highly expressed in the HY strains compared to ...
Thiamine phosphate esters (thiamine monophosphate-TMP; thiamine diphosphate-TDP and thiamine triphosphate-TTP) were measured as their thiochrome derivatives by High Performance Liquid Chromatography in the brains of pyrithiamine-treated rats at various stages during the development of thiamine deficiency enc...
Changes in the amount of thiamine, reduced glutathione, thiamine diphosphate-dependent dehydrogenase activity has been traced after thiamine injection to thiamine-deficient rats and oxythiamine to normal rats. The obtained data show that a drop in reduced glutathione level was a primary reason of the...
Thiamine diphosphate (ThDP) is a cofactor of E1, and it was speculated that the mutations in thiamine-responsive patients involved either the α or the β subunit. It was therefore serendipitous when we found that the E2 subunit in the cell extract from Scriver’s thiamine-responsive patient ...