T4 DNA LigaseT4 RNA Ligase Applications Joining dsDNA with cohesive or blunt termini Joining of ds oligonucleotide linkers or adaptors to dsDNA Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids Ligase-media
赛默飞提供连接酶产品,用于ATP依赖性核酸分子酶促连接。T4 DNA和RNA连接酶是连接酶家族的重要成员,源自T4噬菌体,是克隆实验中最常用的连接酶。
扩增片段长度多态性(AFLP) 连接酶介导的 RNA 检测 双链DNA、 RNA 或 DNA-RNA 杂交双链中缺口的修复 线性DNA 的自我环化 查看原文链接: http://www.thermoscientificbio.com/dna-and-rna-modifying-enzymes/t4-dna-ligase/ 或点击以下阅读原文:
连接酶以ATP-依赖的方式催化核酸分子的连接。T4 DNA和RNA连接酶是连接酶大家族的成员,来自T4噬菌体,在克隆中是最常用的连接酶。 T4 DNA Ligase T4 RNA Ligase 应用 •Joining dsDNA with cohesive or blunt termini •RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate ...
内容提示: PRODUCT INFORMATION T4 DNA Ligase, 5 u/µl #EL0011 Lot Store at -20°C www.thermoscientific.com/fermentas 1000 u Expiry Date Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5??phosphate and 3??hydroxyl termini in duplex DNA or RNA. ...
T4 DNA Ligase中文使用说明 Thermo PRODUCT INFORMATION T4 DNA Ligase, 5 u/µl #EL0011 Lot Store at -20°C 1000 u Expiry Date Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme...
T4�DNA Ligase催化双链DNA或RNA中毗邻的5’-磷酸基团和3’-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口，连接DNA的粘性和平末端，但是该酶对单链核酸无酶活性(1, 2)。T4 DNA Ligase需要辅因子ATP。浓度 1 Weiss u/µl = 200 CEU*/µl 5 Weiss u/µl ...
说明 T4? DNA Ligase催化双链DNA或RNA中毗邻的5’ -磷酸基团和3’-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性 (1, 2)。 T4 DNA Ligase需要辅因子ATP。 浓度 1 Weiss u/μl = 200 CEU*/μl 5 Weiss u/...
T4DNALigase催化双链DNA或RNA中毗邻的5'-磷酸基团和3'-羟基末端之间形成磷酸二酯键 详细介绍 T4 DNA Ligase催化双链DNA或RNA中毗邻的5'-磷酸基团和3'-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性。 T4 DNA ...
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blu...