Ligases catalyze the joining of nucleic acid molecules enzymatically in an ATP-dependent manner. T4 DNA and RNA ligases are members of a large family of ligases and are derived from the T4 bacteriophage representing the most commonly used ligases in clon
T4 DNA Ligase催化双链DNA或RNA中毗邻的5'-磷酸基团和3'-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性。 T4 DNA Ligase需要辅因子ATP。 特点 快速–室温下10分钟内完成粘末端的连接反应。
连接酶以ATP-依赖的方式催化核酸分子的连接。T4 DNA和RNA连接酶是连接酶大家族的成员,来自T4噬菌体,在克隆中是最常用的连接酶。 T4 DNA Ligase T4 RNA Ligase 应用 •Joining dsDNA with cohesive or blunt termini •RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate ...
T4�DNA Ligase催化双链DNA或RNA中毗邻的5’-磷酸基团和3’-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性(1, 2)。 T4 DNA Ligase需要辅因子ATP。 浓度 1 Weiss u/µl = 200 CEU*/µl 5 Weiss u/µ...
说明T4?DNALigase 催化双链 DNA 或 RNA 中毗邻的 5-磷酸基团和 3-羟基末端之间形成磷酸二酯键。酶还可 2、以修复双链 DNA、RNA 或 DNA/RNA 复合体中的单链切口,连接 DNA的粘性和平末端,但是该酶对单链核酸无酶活性(1,2)。T4DNALigase 需要辅因子 ATP。浓度1Weissu/M=200CEU*/日5Weissu/M=1000C...
T4 DNA Ligase, 30 u/µl 5000 u 10X T4 DNA Ligase Buffer 5x1.5 ml 50% PEG Solution5x1.5 ml *Weiss unit Rev.8 V Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme ...
内容提示: PRODUCT INFORMATION T4 DNA Ligase, 5 u/µl #EL0011 Lot Store at -20°C www.thermoscientific.com/fermentas 1000 u Expiry Date Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5??phosphate and 3??hydroxyl termini in duplex DNA or RNA. ...
RNA 结构/功能研究 核糖体 RNA 去除 RNA测序的样品制备 Tools for RNA structure/function research RNA structure is thought to play a central role in many cellular processes, including transcription initiation, elongation and termination, mRNA splicing, and retroviral infecti...
将双链寡核苷酸 linker 或 adaptor 连接到DNA 定点突变 扩增片段长度多态性(AFLP) 连接酶介导的 RNA 检测 双链DNA、 RNA 或 DNA-RNA 杂交双链中缺口的修复 线性DNA 的自我环化 查看原文链接:http://www.thermoscientificbio.com/dna-and-rna-modifying-enzymes/t4-dna-ligase/...
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blu...