E. coli DNA ligase is generally used to eliminate nicks during second-strand cDNA synthesis. Thermo Scientific T4 DNA ligase activity is expressed in Weiss units. One Weiss unit is equivalent to approximately 200 cohesive end units (CEU) which may be used by other vendors. One Weiss unit of...
T4 DNA LigaseT4 RNA Ligase Applications Joining dsDNA with cohesive or blunt termini Joining of ds oligonucleotide linkers or adaptors to dsDNA Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids Ligase-mediated RNA detection Site-directed mutagenesis ...
扩增片段长度多态性(AFLP) 连接酶介导的 RNA 检测 双链DNA、 RNA 或 DNA-RNA 杂交双链中缺口的修复 线性DNA 的自我环化 查看原文链接: http://www.thermoscientificbio.com/dna-and-rna-modifying-enzymes/t4-dna-ligase/ 或点击以下阅读原文:
内容提示: PRODUCT INFORMATION T4 DNA Ligase, 5 u/µl #EL0011 Lot Store at -20°C www.thermoscientific.com/fermentas 1000 u Expiry Date Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5??phosphate and 3??hydroxyl termini in duplex DNA or RNA. ...
连接酶以ATP-依赖的方式催化核酸分子的连接。T4 DNA和RNA连接酶是连接酶大家族的成员,来自T4噬菌体,在克隆中是最常用的连接酶。 T4 DNA Ligase T4 RNA Ligase 应用 •Joining dsDNA with cohesive or blunt termini •RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate ...
T4 DNA Ligase中文使用说明 Thermo PRODUCT INFORMATION T4 DNA Ligase, 5 u/µl #EL0011 Lot Store at -20°C 1000 u Expiry Date Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme...
Double-stranded ligaseKOD RNA ligaseRNA sequencingtemplate dependent ligationthermostable RNA ligaseNicking-sealing RNA ligases play a significant biological role in host defense and cellular repair, and have become an important molecular tool in biomedical engineering. Due to the propensity for RNA to ...
T4DNALigase催化双链DNA或RNA中毗邻的5'-磷酸基团和3'-羟基末端之间形成磷酸二酯键 详细介绍 T4 DNA Ligase催化双链DNA或RNA中毗邻的5'-磷酸基团和3'-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性。 T4 DNA ...
T4�DNA Ligase催化双链DNA或RNA中毗邻的5’-磷酸基团和3’-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口，连接DNA的粘性和平末端，但是该酶对单链核酸无酶活性(1, 2)。T4 DNA Ligase需要辅因子ATP。浓度 1 Weiss u/µl = 200 CEU*/µl 5 Weiss u/µl ...
说明 T4? DNA Ligase催化双链DNA或RNA中毗邻的5’ -磷酸基团和3’-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性 (1, 2)。 T4 DNA Ligase需要辅因子ATP。 浓度 1 Weiss u/μl = 200 CEU*/μl 5 Weiss u/...