Underlay the diluted sample with a volume of Ficoll that is equal to the original sample volume. Centrifuge at 400 xgfor 20 minutes at room temperature with the brake OFF. Harvest PBMC located at the interface of the PBS and Ficoll layers into a fresh tube. ...
Underlay the diluted sample with a volume of Ficoll that is equal to the original sample volume. Centrifuge at 400 x g for 20 minutes at room temperature with the brake OFF. Harvest PBMC located at the interface of the PBS and Ficoll layers into a fresh tube. Fi...