Flag;肽序列DYKDDDDK c-Myc;肽序列EQKLISEEDL 血细胞凝集素 (HA) ;肽序列YPYDVPDYA 绿色荧光蛋白(GFP) 使用标记蛋白的一个缺点是过表达的标记蛋白(而非内源性蛋白)被免疫沉淀,这就限制了这种方法在真正的生物学相关性研究之中的应用。此外,标记蛋白...
solution is added to the beads, which are then mixed and incubated together as a slurry (i.e., the beads are suspended in the solution). At the end of each incubation step, the beads are pelleted to the bottom of the tube ...
Western blot analysis using an anti-FLAG monoclonal antibody demonstrated that all the detergents efficiently solubilized β3AR-cc3.1 (Fig. 2a). Figure 2 Solubilization, purification and monodispersity of β3AR-cc3.1. (a) Western blot (upper panel) and SDS-PAGE (lower panel) analysis of ...
Cells (at an optical density (OD)600 of approximately 9) were pelleted, washed with sterile water, and lysed in 100 μl SDS-sample buffer with 0.5-mm-diameter glass beads (BioSpec Products) by two rounds of boiling for 2 min and vortexing for 30 s. Protein extracts were centrifuged at...
Ligands available Nickel, cobalt Glutathione Anti-DYKDDDDK (FLAG), Anti-c-Myc antibody, or Anti-HA antibody Formats magnetic beads, filter plates, loose resins (magnetic agarose, standard agarose and superflow agarose), spin columns and kits, FPLC cartridges magnetic beads, filter plates, loose res...
Ligands availableNickel, cobaltGlutathioneAnti-DYKDDDDK (FLAG), Anti-c-Myc antibody, or Anti-HA antibody Formatsmagnetic beads, filter plates, loose resins (magnetic agarose, standard agarose and superflow agarose), spin columns and kits, FPLC cartridgesmagnetic beads, filter plates, loose resins (ma...
Fusion tag-binding magnetic beads for IP Tagc-MycFLAGHAHistidineGST Description Consists of a peptide (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc) Immobilized anti-c-Myc antibodies are used to immunoprecipitate c-Myc-tagged recombinant proteins ...
Fusion tag-binding magnetic beads for IP Tagc-MycFLAGHAHistidineGST Description Consists of a peptide (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc) Immobilized anti-c-Myc antibodies are used to immunoprecipitate c-Myc-tagged recombinant proteins Consists of a peptide (DY...
Fusion tag-binding magnetic beads for IP Tagc-MycFLAGHAHistidineGST Description Consists of a peptide (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc) Immobilized anti-c-Myc antibodies are used to immunoprecipitate c-Myc-tagged recombinant proteins Consists of a peptide (DYKDDDDK...
C-terminal DYKDDDDK-tagged Green Renilla Luciferase protein was expressed using the Thermo Scientific 1-Step Human High-Yield Maxi IVT Kit and immunoprecipitated using Pierce Anti-DYKDDDDK Magnetic Agarose or Sigma-Aldrich Anti-FLAG™ M2 Ma...