Deamidation of asparagines is initiated by the cyclization reaction, which occurs between the carboxyl group of the asparagine and amide nitrogen of carboxyl side amino acid (n+1) followed by deamination resulting in the formation of succinimide intermediate. Hydrolysis of the intermediate could occur...
(D. melanogaster) of TOP1 specific substrate and 50 mM NaCl. The reaction was initiated by addition of 2 µL cell extract of either control samples or stress treated samples (see “Preparation of cell extract for molecular analyses”). The samples were incubated at 37 °C (HeLa cells ...