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In this review, Dunlop and Guillemin (2019) describe the requirement to minimize residual serine as a way to maximize the likelihood of observing BMAA misincorporation in vitro. This includes thorough washing of cells using a serine-free medium such as HBSS or PBS prior to adding the treatment...
The cells were then rinsed with PBS, incubated with 100 μg/ml gentamicin (Sigma-Aldrich, G1397) for 10 min and then with a fresh growth medium containing 5 μg/ml of gentamicin. Protocols of the infection and subsequent treatments of the cells are presented in Supplementary Fig. ...
Statistical significance was determined by two-way ANOVA Šídák’s multiple comparison (d, f). Source data are provided as a Source Data file for d, f, h. Source data for a, b can be accessed at DOI 10.17605/OSF.IO/TNAX9. Full size image Environment-mediated drug resistance and ...
Goji (Lycium barbarum) is an important economic crop that is widely cultivated in Northwest China and is known as superfood. Goji plants are often affected by powdery mildew (Arthrocladiella mougeotii) in the field, resulting in considerable reduction in
If it wasn't for the fact that I'm way too lazy to change the channel when I leave the TV on FOX late at night while doing homework, I'd probably have never been exposed to this awful dreck. This show never once strays from one of three premises: Paternity Tests, Rebellious Teens...
The membrane was washed six times in PBS containing decreasing concentrations of SDS (10%, 1%, and 0.1% SDS, applied twice each) for 10 min. The signal of biotin-bound HRP was visualized by 1:5 diluted ECL detection reagent (SuperSignal West Pico PLUS, Thermo Fisher Scientific, 34577). ...
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When processing current sensory inputs, animals refer to related past experiences. Current information is then incorporated into the related neural network to update previously stored memories. However, the neuronal mechanism underlying the impact of mem
Uninfected assay ready compound plates were treated the same way and were used to measure compound cytotoxicity. To monitor the EGFP fluorescence, the plates were transferred to a high-content imager for fluorescence microscopy. A ×5 objective was used to capture one field per well (approximately...