In this review, Dunlop and Guillemin (2019) describe the requirement to minimize residual serine as a way to maximize the likelihood of observing BMAA misincorporation in vitro. This includes thorough washing of cells using a serine-free medium such as HBSS or PBS prior to adding the treatment...
The cells were then rinsed with PBS, incubated with 100 μg/ml gentamicin (Sigma-Aldrich, G1397) for 10 min and then with a fresh growth medium containing 5 μg/ml of gentamicin. Protocols of the infection and subsequent treatments of the cells are presented in Supplementary Fig. ...
A Funny Thing Happened On The Way To The Forum: Comedy Tonight - Zero Mostel And Cast Stop The World - I Want To Get Off: What Kind Of Fool Am I? - Anthony Newley Oliver!: As Long As He Needs Me - Georgia Brown Hello, Dolly!: Hello, Dolly! - Carol Channing And Cast ...
ATG5-deficient, and ATG7-deficient mutant strains at day 5 of chronological ageing measured by PI staining. Data are normalised to the WT Ctrl. at day 1. Comparisons by two-way ANOVA with treatment and strain as independent variables, followed...
A new investigation by National Public Radio (NPR) and the Public Broadcasting Service (PBS)reports that the large oil and gas companies that manufacture plastics have known for decades that recycling plastic was unlikely to ever happen on a broad scale because of the-high costs involved. "They...
If it wasn't for the fact that I'm way too lazy to change the channel when I leave the TV on FOX late at night while doing homework, I'd probably have never been exposed to this awful dreck. This show never once strays from one of three premises: Paternity Tests, Rebellious Teens...
The membrane was washed six times in PBS containing decreasing concentrations of SDS (10%, 1%, and 0.1% SDS, applied twice each) for 10 min. The signal of biotin-bound HRP was visualized by 1:5 diluted ECL detection reagent (SuperSignal West Pico PLUS, Thermo Fisher Scientific, 34577). ...
These mechanisms contribute to the specificity of protein kinase action by providing mechanisms ofa priorisubstrate discrimination. However, another and complementary way to achieve specificity is to increase the signal to noise ratio of protein kinase signalling. Again, non-catalytic functions seem to be...
When processing current sensory inputs, animals refer to related past experiences. Current information is then incorporated into the related neural network to update previously stored memories. However, the neuronal mechanism underlying the impact of mem
Uninfected assay ready compound plates were treated the same way and were used to measure compound cytotoxicity. To monitor the EGFP fluorescence, the plates were transferred to a high-content imager for fluorescence microscopy. A ×5 objective was used to capture one field per well (approximately...