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(Mediatech, Manassas, VA). We cultured SV40 cells in 6-well plates at a density of 1 × 105cells per well for 7 days. The pure ECM secreted by SV40 chondrocytes was obtained by removing cells with ice-cold desoxycholate buffer (0.5% desoxycholate, 1% NP-40, and 150 mM NaCl...
C. muridarum(strain Nigg II) was obtained from the American Type Culture Collection (Manassas, VA) and was grown in HeLa-229 cells73. Elementary bodies (EB) were purified fromC. muridaruminfected cultures using density gradient centrifugation and were stored at −80 °C until used24. The...
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