Nuclear pore complex (NPC) constitutes the only regular route of cargo transport between the cytoplasm and the nucleus.1,2 NPC resides on the nuclear envelope (NE) of all eukaryotic cells. Although the size and composition of the NPC may vary across species,3,4,5,6,7 the overall organizati...
Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop C
Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if...
Mitosis occurs ineukaryotic (animal) cells. Eukaryotic cells have a nucleus that contains the cell’s genetic material. A crucial part of mitosis involves breaking down thenuclear membranethat surrounds the cell’s DNA so that the DNA can be replicated and separated into new cells. Other types ...
Autophagy is a conserved and multipathway-mediated process for organelle degradation in eukaryotic cells [36]. Autophagy is a type of reactive cellular response to changes in the internal and external environment that plays an important role in cell survival and homeostasis [36]. First, an autophag...
Actin is a highly conserved protein that is expressed in all eukaryotic cells and has essential functions in the cytoplasm and the nucleus. Nuclear actin is involved in transcription by all three RNA polymerases, chromatin remodelling, RNA processing, intranuclear transport, nuclear export and in main...
Bestor Eukaryotic cytosine methyltransferases Annu. Rev. Biochem., 74 (2005), pp. 481-514 CrossrefView in ScopusGoogle Scholar 2. M.M. Suzuki, A. Bird DNA methylation landscapes: provocative insights from epigenomics Nat. Rev. Genet., 9 (2008), pp. 465-476 CrossrefView in ScopusGoogle ...
of the nucleotides, whereas those of eukaryotic proteins (for example, the homeodomain family, see 1oct) are parallel to the sugar-phosphate backbone in order to accommodate the longer a helices [22]. Binding by the Trp repressor (1trrA) is unique, with the amino-terminal end of the a ...
In 2011, Ribo-SPIA was first applied to synthesize and amplify cDNA from a single Aspergillus niger cell of eukaryotic microorganisms for single-cell transcriptome analysis [145]. The initial scRNA-seq analysis of prokaryotic cells was conducted in 2015, and the results indicated that ribo-SPIA ...
Chromatin insulators or boundary elements are a class of functional elements in the eukaryotic genome. They regulate gene transcription by interfering with promoter-enhancer communication. The Cp190 protein of Drosophila melanogaster is essential to the function of at least three-types of chromatin insula...