Protein-DNA complexes solved by X-ray crystallography to a resolution of higher than 3.0 Å were obtained from the January 2000 release (04/01/00) of the Protein Data Bank (PDB) [4,5] and the Nucleic Acid Database (NDB) [6]. The complexes were defined as any structure containing one...
AlphaFold can predict the structure of single- and multiple-chain proteins with very high accuracy. However, the accuracy decreases with the number of chains, and the available GPU memory limits the size of protein complexes which can be predicted. Here we show that one can predict the structure...
Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble Article Open access 15 June 2022 Introduction A mechanistic understanding of how a protein’s sequence determines its structural preferences and, ultimately, its biochemical properties is...
Clearly, more structural information is required to better understand the role of the myristoyl moiety of the GCAPs in the course of action of the protein. We have previously studied the structure and dynamics of the myristoyl lipid modification of the GCAP-2 protein bound to lipid membranes [...
Crystal structure of the entire respiratory complex I Nature, 494 (7438) (Feb 28 2013), pp. 443-448 CrossrefView in Scopus [16] M. Cortijo, A. Alonso, J.C. Gomez-Fernandez, D. Chapman Intrinsic protein–lipid interactions. Infrared spectroscopic studies of gramicidin A, bacteriorhodopsin an...
Furthermore, we discovered that TRIM25 mediated the protein arginine methyltransferase 1 (PRMT1)/c-MYC pathway by regulating K63-linked ubiquitination of the oncogene NONO, thereby promoting proliferation and invasion of GBM cells. Materials and methods Cell culture, transient transfection and ...
Cleavage of... Dominguez,Laura,Meredith,... - 《Journal of the American Chemical Society》 被引量: 30发表: 2014年 Analysis of the Overall Structure of the Multi-Domain Amyloid Precursor Protein (APP) The amyloid precursor protein (APP) and its processing by the α-, β- and γ-secretas...
In this work, we have continued our NMR investigations of bL12 by measuring residual dipolar couplings (RDCs) for this protein in the spectrum of intact 70S ribosomes, in order to characterize both the structure of the CTD and the dynamical properties of the hinge region. In particular, we ...
Living systems are driven far from thermodynamic equilibrium through the continuous consumption of ambient energy. In the cell cortex, this energy is invested in the formation of diverse patterns in chemical and mechanical activities, whose spatial and t
Myoglobin, a small oxygen-carrying protein, the first protein to have its three-dimensional structure elucidated (7), was also immediately recognized to need dynamic components for its biological function, as there was no open pathway for the oxygen to get into and out of the protein without so...