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It should be kept in mind that TiO2 is a carcinogen only by inhalation and therefore applications where exposure by inhalation is negligible should not be excluded. Is exposure risk an adequate criterion for the assessment of a secondary material? As shown in Chapter 3, a classification of ...
According to OSHA, these archives should be kept as a record of employee exposure.Searching the Internet is not considered an acceptable method to obtain an SDS, which is why digital and mobile SDS management is so important. Additionally, your employees must have unrestricted access to all SDS...
Cells were lysed in SDS buffer, or 100 µg tissue was homogenized in 4% SDS, 0.1 M DTT buffer. Proteins were precipitated with acetone and redissolved in 8 M urea. The proteins were then trypsin-digested. For SILAC experiments, equal quantities of heavy and light samples were mi...
Western blot Total protein in liver tissue was extracted with RIPA lysis buffer (1 × PBS, 1% Nonidet P-40, 0.5% sodium deoxy- cholate, 0.1% SDS, 1 mM phenyl methyl sulfonyl fluoride and protease inhibitors). Equal protein (80 μg) was ana- lyzed by SDS-PAGE electrophoresis,...
Specifically, sections were air-dried for 15–20 min and fixed in 3.7% paraformaldehyde in PBS for 10–15 min, followed by a 2 min incubation in 4% SDS in PBS and washes with PBS. The fixed sections were then incubated in 70% ethanol at 4 °C for at least 16 h. Next...
The immunocomplexes were extracted from the beads by the treatment with a sample buffer for SDS-PAGE (95 °C, 5 min), and analyzed by Western blot with anti-MMP-2 HPX antibody or anti-Xenopus sperm-surface glycoprotein (SGP) antibody (2A3D9, Nagai et al., 2009). 4.3. Assay for...
The beads were washed five times with lysis buffer, and the proteins were eluted using denaturing sodium dodecyl-sulfate (SDS) sample buffer. Cell lysates were separated using SDS polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA)...
By qRT-PCR, we validated the SDs in 11 BACs that were determined by WSSD strategy (Table 1). The lengths of these SDs in the BACs range from 11 kb to 13 kb and the corresponding reads depths are listed in Table 1. Previous studies showed that the copy number of SDs should be less...