Human RPE cells (ARPE-19, CRL-2302, American Type Culture Collection, Manassas, VA; distributed by LGC Standards GmbH, Wesel, Germany) were routinely cultured in a mixture of Dulbecco’s modified Eagle’s medium (DMEM)/F-12 with GlutaMAX (31331028, Thermo Fisher Scientific), supplemented with...
The Cas9-sgAi9 group exhibited 0.51% of reads containing indels, confirming a 5.94-fold higher RPE editing compared to the control group (Fig. 5H). Overall, the results indicated that Cas9-sgAi9 delivered by LNPx mediated gene editing in the retina, with the most robust editing observed in...
Human glioblastoma type IV cell line U87-MG (ATCC® HTB-14™) and Eagle’s minimum essential medium (EMEM) were purchased from ATCC© (ATCC©, Manassas, VA, USA). Phosphate buffered saline (PBS), EMEM powder (Gibco, Monza, Italy) and fetal calf serum (FCS, Invitrogen, Monza, ...
The diffusion of viruses at the cell membrane is essential to reach a suitable entry site and initiate subsequent internalization. Although many viruses take advantage of glycosaminoglycans (GAG) to bind to the cell surface, little is known about the dyn
I bought a niceSamsung 500GB SSDrecently to replace the ol’ spinning disk that came with my 2010 15″ MacBook Pro (Snow Leopard). Pricing had finally come down to reasonable levels ($280) and this helps stave off my itch to upgrade (eyeing the 13″ Retina MBP). ...
By submitting this form, you are consenting to receive marketing emails from: Villagio Hospitality Group, 9901 Discovery Blvd, Manassas, VA, 20109, US, http://www.villagiogroup.com. You can revoke your consent to receive emails at any time by using the SafeUnsubscribe® link, found at th...
The 3T3 cells (ATCC, Manassas, VA) were cultured in Dulbecco’s modified eagle’s medium (DMEM) supplemented with 5% fetal bovine serum (FBS) (Invitrogen, Grand Island, NY), 2 mM glutamine (Invitrogen), 100 U/mL penicillin (Invitrogen), and 100 μg/mL streptomycin (Invitrogen) at...
(OMEGA-3). DHA is mainly found in the brain and retina of the human body and has important physiological functions such as promoting nervous system development, improving retinal function, improving vision, preventing cardiovascular disease, treating cardiovascular disease, resisting inflammation and ...
Discover apartments in Manassas, VA with attractive apartment interiors and relaxing outdoor amenities. Speak to a leasing consultant today!
All of the cell lines were obtained from the American Type Culture Collection (ATCC) in Manassas, VA except the AGS cells (gift of Dr. Anil Rustgi, University of Pennsylvania). Cells were maintained in flasks (37°C) in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal...