The tail was reconstructed using the Rosetta macromolecular modelling platform58, with a substantial part of the protocol implemented in PyRosetta78. Throughout the protocol, we have used the standard dual space energy function of Rosetta79, permitting for non-ideal protein geometry. The molecular ...
Download all Video S1. Structural Model of the Endogenous BAF Complex, Spin View, Related to Figures 1, S1, S2, and S3 Video S2. Interfaces of the BAF Complex with the NCP, Including Interfaces of the ATPase Module with the NCP (Part 1) and the SMARCB1 Subunit with the NCP (Part ...
Activity of αGal was assayed using the synthetic substrate, 4-methylumbelliferyl-α-d-galactopyranoside (MUG) as described (Chen 2000) with modifications to a microtiter plate format (below). Enzyme activity is measured in units/ml where one unit is defined as the amount of enzyme required to ...