The biomass was further washed off with 1 mL of the cooled solution of 0.85% sodium chloride (pH 7.2) added to each tube. The obtained microbial suspension of B. anthracis 55ΔTPA-1Spo– strain was transferred to the Hottinger’s agar slant tubes containing kanamycin (25 μg/mL) and ...
For this purpose spores were transferred using sterile needle from a 05 day old fungal culture grown on PDA slant and re-suspended in 10.0 ml sterile distilled water containing 0.1% Tween-20. Each suspension was serially diluted up to 10-5 in order to make homogenous spore suspension of 106...
The stock culture was maintained on nutrient agar slants kept at 4 oC. Seed culture was prepared by transferring a full loop of cells from the slant culture into 50 ml of medium (250 ml Erlenmeyer) composed of (g/l): glucose, 20; NH4Cl, 2.5; yeast extract, 1; KH2PO4, 0.3; NaCl,...
The purpose of this study, was to confirm the findings described above in Example 5 and to extend the scope of the investigation. Field trials were carried out in two major grape growing regions of New Zealand, Hawkes Bay and Marlborough, to determine the potential impact of regional and seas...
The invention relates to a process of manufacturing D(-)-3-hydroxybutyric acid by breeding microorganisms capable of producing said acid in a nutrient medium containing certain specific carbon source;
The mixed culture was stored on agar slants and in lyophilized or liquid broths. The broth was most satisfactory for routine experiments as cell growth and viscosity reduction occurred in 1-3 days vs 2-3 wks when started from agar slant or lyophil cultures. ...
The microorganism is inoculated from an agar slant culture into 500 ml of cultivation medium with the use of the customary microbiological working procedures and incubated at 30° in a sterile Erlenmeyer flask with sterile stopper during 48 h on a rotary shaking machine. This batch is then ...
An inoculum of Streptomyces thermoarchaensis NCIB 12015 was prepared by washing with sterile water (10 ml) a slant culture which was maintained on a medium prepared from yeast extract (0.5 g), malt extract (30 g), peptone (5 g) and agar (20 g) in tap water (1 liter adjusted to pH...
Streptomyces sp 142 (FERM P-10806), isolated from a soil sample, produced lacto-N-biosidase when grown on a medium containing L-fucose as the sole carbon source as described below. Spores and mycelia of the strain grown on an agar slant were used to inoculate 100 ml of a medium contain...
The yeast strain is prepared to form a footing for seeding the fermenters by starting propagation every day at the distillery laboratory from an agar slant containing a pure culture of the yeast. 35 By known methods of bacteriological technique, a portion of this pure culture is transferred to...