Learn how to perform a Gram stain, and see the purpose of Gram staining. Know Gram stain steps, and a description of what happens at each step in the Gram stain. Related to this QuestionWhy is a gram stain helpful when monitoring bacterial contamination in food or inve...
(grăm)alsoGram's stain(grămz) n. A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranin. Because of differences in cell wall structure...
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Another potential mechanism through which diet could increase inflammation within the hypothalamus involves the gut microbiota. It has been proposed that high-fat diets alter the composition of the gut microbiota, which in turn leads to increased intestinal permeability and systemic exposure to bacterial ...
(Supplementary Fig.S2). To our knowledge this phenotype has not previously been reported with f1ori-containing plasmids. The purpose of the f1oriin the pEGFP plasmid is to facilitate ssDNA replication and phage packaging [53,60]. The f1oriis therefore likely an artefact due to the prior use...
Finally, the STX medium was developed with the purpose of correcting problems observed in the RSA medium (Sant'Ana and Azeredo, 2005, Schoeller and Ingham, 2001). Although STX is a new version of Petrifilm™ plates for Staphylococcus enumeration, several colonies with atypical morphology on ...
The microflora of the penile skin-lined neovagina in male-to-female transsexuals is a recently created microbial niche which thus far has been characterized only to a very limited extent. Yet the knowledge of this microflora can be considered as essential to the follow-up of transsexual women. ...
Indeed, the purpose of this system is to play the role of an alarm signal when the models obtained over time move away from the “normal” state. The normal state is determined by the collected data and by creating “baselines” for a given area or community (Wang et al., 2005, ...
To determine whether and how microglia modulate sleep, we used a transgenic mouse line in which the diphtheria toxin (DT) receptor is conditionally expressed in microglia for the purpose of microglial depletion27,28. We found that microglial depletion dramatically decreased stable wakefulness due to ...
the purpose of our studies we used this to polarize our endothelial layers as standard. LPS was used as our in vitro endotoxin injury (where live Gram-negative bacteria use was not feasible) and has been shown to directly increase E-selectin and integrin counter receptor expression41. The ...