contortus by immunofluorescence The prokaryotic expression protein, Hc-PBS-5 (284 aa, together with a SUMO tag, ~ 43.2 kDa), was purified and identified, as shown in Fig. 2b, and its rabbit-derived polyclonal antibody was produced. We observed that positive-serum antibody could bind ...
The microbial DNA was extracted by the E.Z.N.A. Stool DNA kit (Omega inc., USA) and the total DNA was eluted in 50 μL of elution buffer, stored at -80℃ until measurement in the PCR (LC-Bio Technology Co., Ltd., China). The V3-V4 region of the prokaryotic 16S rRNA gene ...
However, the absence of ApcH and CpcN homologs in crown Cyanobacteria implies that CpcC and CpcG, rather than CpcN and ApcH, are favored for PBS assembly in cells with thylakoid membranes (Figs. 4a and 5a). Surprisingly, other than Aurora, we found no homologs of ApcH and CpcN (identity ...
624 single-copy core genes from their genomes. Phylogroups are indicated by shaded colors. The symptom status of the host for each isolate is indicated by the color of the isolate name
Vertebrate AANATs also have high selectivity for arylalkylamines conferred by the binding pocket. Non-vertebrate type AANATs are found in the genomes of most fungi, many unicellular eukaryotes, and a variety of bacteria [16]. The proteins encoded by these genes do not contain the characteristic...
Repetitive DNA sequences (repeats) are patterns of nucleic acids that occur in multiple copies throughout the genome1. Both eukaryotic and prokaryotic organisms contain a certain proportion of repeats in the genome2,3,4, particularly mammalians, in which repeats account for 25–50% of their enti...
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We first investigated the secondary metabolic potential of marine prokaryotes by analyzing the diversity and novelty of the biosynthetic gene clusters (BGCs) in 7541 prokaryotic genomes from cultivated and single cells, along with 26,363 newly assembled medium-to-high-quality genomes from marine environ...
(http://www.prodoric.de/JCat), three of the provided additional options were availed for avoiding the termination of the rho-independent transcription, binding site of the prokaryotic ribosome and cleavage sites of restriction enzymes. The output received from the tool consists of a codon ...
Predatory bacteria feed upon other bacteria in various environments. Bdellovibrio exovorus is an obligate epibiotic predator that attaches on the prey cell surface, where it grows and proliferates. Although the mechanisms allowing feeding through the pre