The process of making RNA from DNA is called what? DNA to RNA DNA contains the information that all of our cells need to create necessary proteins and function normally. However, DNA cannot leave the nucleus of the cell, and so RNA must be created, using DNA as a template, so that the...
The expression of the apoptosis-related gene BCL2L13, XAF1, and NF2 were significantly different between OA and healthy meniscus samples. The construction of a covariation network of RNA-binding proteins (RBPs) and RAS genes revealed that differentially expressed RBP genes LAMA2 and CUL4B may ...
Protein lysates were prepared from cells using RIPA lysis buffer (R0010, Solarbio) containing protease and phosphatase inhibitors (P1260, Solarbio). The protein concentration was determined via a BCA protein assay (CW0014S, CWBIO). Proteins were separated by 10% or 12% SDS‒polyacrylamide gel ...
Our current food system relies on unsustainable practices, which often fail to provide healthy diets to a growing population. Therefore, there is an urgent demand for new sustainable nutrition sources and processes. Microorganisms have gained attention a
Therefore, precise analysis of the process of somatic cell reprogramming at the single-cell level is of great potential value for improving our understanding of the regulatory mechanism of plant regeneration. Recent advances in single-cell RNA sequencing (scRNA-seq) provide an unprecedented opportunity...
of mRNA vaccines [7]. mRNA vaccination has become a more practical choice as a result of significant technological developments that have taken place over the last several decades [11]. The RNA may be made more resistant to RNases, more stable, and more translation-friendly by making various...
and M.J. performed cloning, purification and spectral analysis of the different DPH proteins with the assistance of J.S.; C.D. and M.J. generated the GFP reporter lines; C.D. performed the induction and reversion spectra with support from J.S.; M.J. generated resources (complementation ...
AlkB homolog 5 (ALKBH5) and fat mass and obesity-associated (FTO), selectively remove this methylation, making m6A modification dynamic and reversible. In addition, m6A “readers”, which are RNA-binding proteins such as YTH domain-containing (YTHDC) proteins, insulin-like growth factor 2 mRNA...
Fasting is associated with a range of health benefits1–6. How fasting signals elicit changes in the proteome to establish metabolic programmes remains poorly understood. Here we show that hepatocytes selectively remodel the translatome while global tran
RNA-binding proteins (RBPs) modulate alternative splicing outcomes to determine isoform expression and cellular survival. To identify RBPs that directly drive alternative exon inclusion, we developed tethered function luciferase-based splicing reporters