待解决 悬赏分:1 - 离问题结束还有 cDNA was generated using the PrimeScript™ RT reagent kit问题补充:匿名 2013-05-23 12:21:38 基因是使用PrimeScript ™ RT试剂盒产生 匿名 2013-05-23 12:23:18 使用PrimeScript™ RT试剂成套工具, cDNA引起了 匿名 2013-05-23 12:24:58 cDNA使用PrimeS...
USA). Template cDNA was synthesized using the Primescript RT reagent kit with gDNA eraser (Takara, Tokyo, Japan). Real-time quantitative PCR was performed using the following primers:
Samples were harvested and ground into a fine powder using liquid nitrogen. Total RNA was extracted using RNAiso reagent (BIOFIT, RN33050) as recommended by the manufacturer. One microgram of RNA was used for first-strand cDNA by the PrimeScriptTM RT reagent Kit containing gDNA eraser (Takara,...
The PrimeScript Double Strand cDNA Synthesis Kit contains all of the reagents necessary to synthesize double-stranded cDNA from purified poly A+RNA templates using the Gubler-Hoffman method (Gubler and Hoffman 1983). First-strand cDNA is synthesized using PrimeScrip...
All samples were homogenized in TRIzol Reagent (Invitrogen, 15596018). RNA from adipose tissues was isolated according to the manufacturer’s protocol. For each sample, 1 μg of total RNA was reverse transcribed by using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). For qPCR,...
Total RNA was isolated from tissues or cultured cells using Trizol reagent (Invitrogen) according to the manufacturer’s protocol. One microgram of total RNA was reverse transcribed in a final volume of 20 μl using PrimeScript RT Master Mix (Takara, Dalian, China). qRT-PCR was performed as ...
According to the manufacturer’s instructions, 2 μl RNA sample was reversely transcribed to cDNA using PrimeScript® RT reagent Kit (DDR047A, Takara). Primer pairs for qRT-PCR analysis (Additional file 5: Table S3-D) were designed by the Primer3Plus program (http://www.bioinformatics.nl...
cDNA was synthesised from total RNA with the PrimeScript RT reagent kit (Takara Bio, Shiga, Japan). Quantitative polymerase chain reaction (PCR) was performed using the SYBR Premix ExTaq2 kit (Takara Bio) with Rplp0 as the control gene. The primer sets are listed in Supplementary Table 2....
mice, Total RNA was extracted using Trizol Reagent. followed by reverse transcription to complementary DNA (cDNA) using the PrimeScript RT Reagent Kit (Takara, Japan). To quantify mRNA expression, cDNAs were amplified by RT-qPCR with the SYBR Premix Ex Taq RT-PCR kit (Takara, Japan) using ...
Total RNA was extracted using TRIzol reagent (TaKaRa, Shiga, Japan), and cDNA was synthesized using the PrimeScript RT–PCR Kit (TaKaRa, Shiga, Japan). Real-time quantitative PCR was performed using SYBR Green Master Mix (TaKaRa, Shiga, Japan) on a LightCycler 96 Detection System (Roche, Ma...