Protein concentration was quantitated and determined using the PierceTM BCA Protein Assay Kit (Thermo Scientific, US). Equal amounts of protein were loaded and separated by SDS-PAGE using Mini-Protean TGX polyacrylamide gels (Bio-Rad). Proteins were then transferred onto PVDF membranes, blocked ...
The Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) was used to determine the total protein concentration in the samples. 50 µg of proteins was separated using the 4–20% and 6% Novex Wedge Well Tris–Glycine gels (Invitrogen, Carlsbad, CA, USA). After ...
Protein pellets were dissolved in 1% sodium dodecyl sulfate (SDS). Protein concentrations were quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and measured with a CLARIOstar plate reader (BMG Labtech) using a bovine serum albumin (BSA) dilution series as standard. ...
The supernate was transferred to 1.5 mL EP tube and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific Rockford, USA). Thirty micrograms of tissue lysates boiled with 5 × loading buffer (Beyotime, China) were loaded onto 10% or 12.5% SDS–...
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Protein content was verified by the Pierce method (Pierce BCA Protein Assay Kit, cat. no. 23,227). An equal amount of protein was precipitated by the addition of 1:1 (v/v) of 10% trichloroacetic acid (TCA) and incubated for 20 min on ice. Samples were spun for 10 min at 20 000...
The protein concentrations were measured using the Pierce® BCA Protein Assay Kit (Thermo Scientific) according to the manufacturer’s instructions. Gel electrophoresis and blotting were performed using the NuPAGE system from Invitrogen according to the manufacturer’s instructions; as previously ...
Overview of Protein-Protein Interaction Analysis Select products Pierce Classic Magnetic IP/Co-IP Kit Pierce Direct Magnetic IP/Co-IP Kit Pierce Crosslink Magnetic IP/Co-IP Kit Dynabeads Co-Immunoprecipitation Kit Protein Interaction Analysis...
The protein concentration was determined using the Pierce™ BCA protein assay kit following manufacturer’s instructions (Thermo Fisher Scientific, Massachusetts, USA). Each sample was separated by non-reducing SDS-PAGE with Spectra™ Multicolour Broad Range Protein Ladder (Thermo Fisher Scientific, ...
Protein concentration was determined using the Pierce™ BCA Protein Assay Kit (cat.#23225, Thermo Scientific). Total protein extracts (20 µg) were resolved in NuPAGE™ 4–12% Bis-Tris Protein Gels (cat.#NPO335BOX, Invitrogen) by electrophoresis and then transferred to polyvinylidene ...