The development of the patch-clamp technique in the late 1970s has given electrophysiologists new prospects. It allows high-resolution current recordings not only of whole cells, but also of excised cellular patches. Even single-channel opening events can be investigated. However, with its complex...
This article gives an introduction to the patch-clamp technique and how it is used to study the physiology of ion channels for neuroscience and other life-science fields.
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This paper describes a method to obtain isolated photoreceptors by means of enzymatic and mechanical dissociation of the whole retina of Orconectes limosus and the application of patch‐clamp technique to these dissociated ommatidia. Several gigaseals and single‐channel currents were recorded, ...
SINGLE ION CEANNELS THE PATCH-CLAMP TECHNIQUE USED TO EXAMINE THE OPERATION OF SINGLE MEMBRANE MCLECULES FORMING SINGLE ION CEANNELSTHE PATCH-CLAMP TECHNIQUE USED TO EXAMINE THE OPERATION OF SINGLE MEMBRANE MCLECULES FORMING SINGLE ION CEANNELSdoi:10.1111/j.1467-2995.1983.tb00032.x...
Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism ...
R., and Stühmer, W., 1983a, Lateral distribution of sodium and potassium channels in frog skeletal muscle: Measurements with a patch clamp technique,J. Physiol. (Lond.) 336:261–284. CAS Google Scholar Aimers, W., Stanfield, P. R., and Stühmer, W., 1983b, Slow changes in ...
Patch-clamp is one of the most indispensable techniques in the study of neuroscience, especially in the field of neurophysiology. Here, we describe the experimental details using the whole-cell patch clamp mode in the study of nanoparticles in hippocampal slices of the rat, including the ...
The blind patch-clamp method displays its greatest power in the experiments in which long-term, stable recordings are required, such as those for the analysis of long-term potentiation (LTP) in hippocampal slices. Relatively thick slices, which have less damage during preparation, can be used ...
not available for EP1019718of corresponding document: US6379916 Disclosed is a device and a process for examining cells using the patch-clamp method. A plane arrangement is used of a first number of micro-cuvettes for receiving cells and a plane arrangement of a second number of micro-pipettes...