The p53 protein is a transcription factor involved in processes of cell growth and differentiation. The muscle creatine kinase (MCK) gene whose transcription is induced during muscle differentiation contains p53-binding sites. In this study we tested the involvement of p53 in the activation of MCK ...
Transcriptome and proteome profiling revealed that the antitumor activity of ginsenosides is closely associated with P53 protein. A miRNA–proteome interaction network revealed that P53 controlled the transcription of at least 38 proteins, and proteome-metabolome profiling analysis revealed that P53 regulated...
p53 is a nuclear protein that functions as a regulator of transcription. Significant advances have been made recently in our understanding of how p53 function is regulated and the mechanisms by which p53 mediates its effects. Introduction p53 has been the subject of intense research activity over ...
p53 is a tumour-suppressor protein that induces apoptotic cell death in response to oncogenic stress. Malignant progression is dependent on loss of p53 function, either through mutation in the TP53 gene (which encodes p53) itself or by defects in the signalling pathways that are upstream or down...
14-3-3 proteins play diverse and important roles in the regulation of wild-type p53 activity. 14-3-3σ has distinctive roles and engages in non-canonical interactions with partner proteins. Selective modulation of 14-3-3 protein–protein interactions, via stabilisation or inhibition, is a new ...
However, the role of the wild-type p53 gene in growth control is not known. Hybrid proteins that contain the DNA binding domain of yeast GAL4 and portions of p53 have been used to show that the p53 protein contains a transcription-activating sequence that functions in both yeast and ...
Wild-type but not mutant p53 protein bound to oligonucleotides corresponding to this region of the bax promoter, based on gel retardation assays. Taken together, the results suggest that bax is a p53 primary-response gene, presumably involved in a p53-regulated pathway for induction of apoptosis....
The prime mechanism by which p53 acts as a tumor suppressor is as a transcription factor regulating the expression of diverse downstream genes. The DNA-binding domain of p53 (p53DBD) interacts with defined DNA sites and is the main target for mutations in human primary tumors. Here, we show...
In its essence, p53 is a stress-responsive transcription factor. Upon activation in response to a diverse array of stressors, the tetrameric form of the protein binds to DNA in a sequence-specific manner [6]. Once bound to DNA, p53 activates a range of antiproliferative programs, as well ...
S. Fields et al, "Presence of a Potent Transcription Activating Sequence in the p53 Protein", Science, 249:1046-1049 (Aug. 31, 1990). J. Bargonetti et al, "Site-Specific Binding of Wild-Type p53 to Cellular DNA is Inhibited by SV40 T Antigen and Mutant p53", Genes & Development,...