we performed an unsupervised pathway enrichment analysis. For each metabolite with at least one GMI, we investigated whether specific pathways and processes were over-represented in the transcripts correlated with that metabolite. Overall, the considered transcripts spanned over ...
This was accompanied by a higher resistance to the inhibition of mitochondrial respiration by antimycin A, a phenotype confirmed in Roxadustat-treated Huh7 cells and Molidustat-treated hepatoblastoma cells (Huh6 and HepG2). Overall, this study shows that HIF-inducing drugs increase the metabolic...
The reaction was stopped by addition of an equal volume of serum-containing DMEM high glucose medium. Cells were collected, centrifuged at 300 × g for 5 min and the cell pellet resuspended in DMEM high glucose supplemented with 10% heat-inactivated FCS and 1% P/S and counted using ...
5c, d). The axial ligand coordination reaction of the c-hemes with AcMet has been demonstrated to increase the reduction potential (E0ˊ) of c-type cytochromes to cause the formation of a large energy barrier for the electron transfer process29,30. This AcMet coordination could thus block ...
However, with increased concentrations of ATP or if respiration is compromised and the pmF falls, the ATP synthase reaction can reverse and hydrolyze ATP to pump protons back out of the matrix to re-establish the pmF. Ultimately the efficiency of the OXPHOS system is influenced by electrochemical...
An effective long PCR-based method has been established for the amplification and subsequent sequencing from individuals via primer walking [14]. However, it has not yet been possible to directly sequence an entire mt genome sequence in a single reaction. With the recent focus on the sequencing ...
LIAS has two [Fe4–S4] clusters, which are essential for the enzymatic reaction as one of them provides sulfur for sulfurylation of octanoate to lipoate, during catalysis [252]. Lipoic acid defects were found in four syndromes caused by mutations in NFU1 (MMDS1, multiple mitochondrial ...
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X). First strand cDNA was synthesized using random hexamer primers and MMuLV Reverse Tr...
Thirty nanograms of cDNA and 50 nM of the paired primer mix for target genes were used for each reaction. Relative mRNA was normalised to the housekeeping gene act-1 (Supplementary Table 1). Oligonucleotides used in this study are listed in Table S2. Statistics Error bars indicate standard...
The overall reaction allowing for a varying amount (denotes by coefficient n) of ATP produced is given by: $$\begin{array}{c}{\rm{G}}{\rm{l}}{\rm{u}}{\rm{c}}{\rm{o}}{\rm{s}}{\rm{e}}+2\,{\rm{N}}{\rm{A}}{\rm{D}}{({\rm{P}})}^{+}+{\rm{n}}\,{\rm{A...