Biotechnology — Nucleic acid synthesis — Part 1: Requirements for the production and quality control of synthesized oligonucleotidesdoi:ISO 20688-1:2020本文件规定了合成寡核苷酸(名义上最多250个碱基)的生产和质量控制的最低要求.\n本文件还描述了合成寡核苷酸的一般质量属性以及评估质量属性的常用方法....
We report the design and synthesis of an orthogonally protected peptide nucleic acid (PNA) building block, Fmoc-PNA-U'-(Dde)-OH, and its use in the construction of PNA FRET probes. This building block allows for the post-synthetic attachment of reporter groups to the amino group attached to...
4). At the beginning of the simulation, the peptide in model 6 changed the orientation of the amino acid side chains towards a clear amphipathic conformation in space: the hydrophobic amino acid residues were oriented towards the inner membrane and the polar amino acid residues were facing the ...
Predatory bacteria feed upon other bacteria in various environments. Bdellovibrio exovorus is an obligate epibiotic predator that attaches on the prey cell surface, where it grows and proliferates. Although the mechanisms allowing feeding through the pre
GSH is a tripeptide composed of the amino acids glutamic acid (Glu), cysteine (Cys), and glycine (Gly), and serves as a crucial intracellular antioxidant [54]. A decline in GSH synthesis disrupts the intracellular redox balance, leading to the accumulation of peroxidized PUFAs. This inability...
Reutilization of both hypoxanthine and xanthine for nucleotide and nucleic acid synthesis is markedly enhanced when their oxidations are inhibited by allopurinol and oxipurinol. This reutilization does not disrupt normal nucleic acid anabolism, however, because feedback inhibition is an integral part of ...
Conclusion These and other data demonstrate that the N-terminal region of eRF3 is involved both in modulation of the efficiency of translation termination and functioning of the eRF1/eRF3 complex outside of translation termination.Background Termination of protein synthesis takes place in ribosomes ...
Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods 07 April 2021 Background Anther development is crucial for male fertility and sexual reproduction in plants [1]. Cells in an anther undergo meiosis to produce microspores, which...
Retinoic acid signaling is critical during the totipotency window in early mammalian developmentAne Iturbide, Mayra L. Ruiz Tejada Segura, Camille Noll, Kenji Schorpp, Ina Rothenaigner, Elias R. Ruiz-Morales, Gabriele Lubatti, Ahmed Agami, Kamyar Hadian, Antonio Scialdone & Maria-...
The extent of such selectivity becomes evident in processes in which complementary strands have to selectively pair amid a plethora of other nucleic acid polymers and oligomers. Relevant examples of such a successful “needle in the haystack” search performed by nucleic acids can be found in both...