to experience to explain the object to explore the possib to export category ii to express disagreeme to express opposition to express pleasure to extend ones deepes to face the unthinkab to facilitate this to fail eg experiment to fall into to fall short of the to fancy to feel comfortabl...
in the rural regions in the same imprinted in the same manner wa in the script in the second instanc in the shadow of the in the short to long in the sitting room in the specific instr in the spell descript in the sping becomes in the sports aspect in the street under t in the sup...
Cyberpunk is a sci-fi subgenre born in and very much about the 1980s, but it's started to feel distressingly relevant again in recent years. It's both an aesthetic—all neon light and rain-slick streets at night—and an ethos. A reaction to the kind of sci-fi more concerned with...
The upper phase was transferred to a new 2-ml reaction tube. Then 1 ml of diethyl ether was added to the tube with the lower phase and a second agitation/centrifugation/transfer step was performed. 250 µl of each sample were then distributed into GC vials in triplicates and 25...
He spends the season living in a trailer and working the night shift, until a mysterious woman and some colorful customers rescue him from self-destruction. Cast: Kentucker Audley, Hannah Gross, Jason Shelton, Oona Roche. North American PremiereCronies / U.S.A. (Director and screenwriter: ...
(Invitrogen) was used to generate cDNA; the reaction was primed to incorporate a specific tail for subsequent PCR. The resulting cDNA from each animal was then pooled and used to amplify the total VH and VLK gene set. The PCR was performed at 68 °C using KOD polymerase (Thermo ...
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- When bKnockedDown and !hasHitReaction and !bOnFloor and playerAttackPosition = FRONT/BACK, Zed now goes to staggerback-knockeddown. - When playerAttackPosition is not FRONT/BACK, Zed goes to falldown. - attack-network no longer goes to idle when bDead. ...
4a-v). The samples were rotated several times during UV exposure to ensure a homogeneous reaction throughout the template. The implants were released by mechanically agitating the templates in isopropyl alcohol (IPA) using a sonicator (DT 100 H, Bandelin Sonorex Digitec, Germany) (Supplementary ...
of the purified nicked minicircle was incubated with T4 ligase (New England Biolabs) and ligase buffer containing 25 µg mL−1 bovine serum albumin, in the presence of different quantities of ethidium bromide (EtBr) in a total reaction volume of 3 mL, at room temperature overnight....