Protein–protein interactions (PPIs) have pivotal roles in life processes. The studies showed that aberrant PPIs are associated with various diseases, including cancer, infectious diseases, and neurodegenerative diseases. Therefore, targeting PPIs is a direction in treating diseases and an essential strate...
The insert shows the integrated intensity of the peaks as function of aggregation time, corresponding to depletion of monomers and possibly of small, mobile oligomers. Full size image To discriminate between these possibilities, we performed negative stain TEM. As shown in Fig. 3D, hIAPP forms ...
Corresponding changes in the peak width were observed, with a similar peak width for the monomers at (108 ± 1) photons and an increased peak width for dimers at (175 ± 13) photons. From the peak integrals, a dimerization level of ~18% was estimated. TpoR monomers and dimers...
A related approach was used for the design of small-molecule binders6; as protein targets are so much bigger and because nonpolar interactions are the primary driving force for protein–protein interactions, we focused the RIF generation process on nonpolar sites in specific surface regions of ...
Lane 3, boiling of the oligomerized sample with SDS results in dissociation of the heptamers into monomers. The 129YTRF Mutation Exerts a Dominant Negative Effect upon Membrane Insertion of Wild Type Toxin into Fibroblast Membranes To learn more about the functional properties of the129YTRF ...
Interestingly, residues 45–53 and 283–286 of Omp32 (which contain T1 and T7) contribute to a network of polar and nonpolar interactions between the interfaces of adjacent monomers. These clusters of amino acids are almost invariable (Figure 8) and might therefore have an essential role in ...
The lamins are the major architectural proteins of the animal cell nucleus. Lamins line the inside of the nuclear membrane, where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. They have been implicated
(R2 = 0.94,p < 2.2e−16) with the number of centromeric monomers (Additional file 2: Fig. S16B). Moreover, 25 intact LTR retrotransposons were identified across the centromere regions, with over 80% as Gypsy class. The majority of these retrotransposons showed high sequence ...
et al. Critical role of interfaces and agitation on the nucleation of Aβ amyloid fibrils at low concentrations of Aβ monomers. Biochim. Biophys. Acta Proteins Proteom. 1804, 986–995 (2010). Article CAS Google Scholar Gray, J. J. The interaction of proteins with solid surfaces. Curr....
Protein solubility and stability depend on the co-solutes present. There is little theoretical basis for selection of suitable co-solutes. Some guidance is provided by the Hofmeister series, an empirical ordering of anions according to their effect on so