Journal of Biological ChemistryMiles, E. W.; Rhee, S.; Davies, D. R. The molecular basis of substrate channeling. J. Biol. Chem., 1999, 274, 12193-12196Miles, E.W., Rhee, S. and Davies, D.R. (1999) The molecular basis of substrate channeling. J. Biol. Chem. 274, 12193±...
R. The Molecular Basis of Substrate Channeling. J. Biol. Chem. 274, 12193–12196 (1999). Article CAS Google Scholar Rocha-Martín, J., Rivas, Bdelas, Muñoz, R., Guisán, J. M. & López-Gallego, F. Rational Co-Immobilization of Bi-Enzyme Cascades on Porous Supports and their ...
This exposure may facilitate downstream enzymes like PGAP5 to bind the alkyl chain (along with the exposed glycan), potentially aiding in substrate channeling. Given that PLM remains bound to cPGAP1 during purification and is enclosed by GPI-AP2 in the guitar-shaped cavity (Fig. 4b), it is...
Both pigments (anthocyanins and betalains) were never observed together in the same plant which resulted in the mutual exclusion theory. A molecular mechanism which could cause this mutual exclusion is not known yet. Betalains do not occur in plant species outside the Caryophyllales. The current kn...
An analysis of a recently reported crystal structure of the WT-R132H IDH1 heterodimer and of in vitro kinetic parameters for 2-HG production, however, indicated that substrate channeling between the subunits is biophysically implausible. We also found that putative carbon-substrate flux between WT ...
Directional regulation of enzyme pathways through the control of substrate channeling on a DNA origami scaffold. Angew Chem Int Ed. 2016;55:7483–6. CAS Google Scholar Yin L, Guo X, Liu L, Zhang Y, Feng Y. Self-assembled multimeric-enzyme nanoreactor for robust and efficient biocatalysis. ...
amino acid linkers. The overall reaction rate was much higher in the reaction catalyzed by the fusion enzyme than in the reaction catalyzed by the native non-fused enzymes (Iturrate2009). However, substrate channeling in fusion proteins may also be absent. An exploration of the kinetics of ...
BphJ, a nonphosphorylating acylating aldehyde dehydrogenase, catalyzes the conversion of aldehydes to form acyl-coenzyme A in the presence of NAD+ and coenzyme A (CoA). The enzyme is structurally related to the nonacylating aldehyde dehydrogenases, aspartate-β- semialdehyde dehydrogenase and ...
Imidazole glycerol phosphate synthase (HisFH) is a heterodimeric bienzyme complex operating at a central branch point of metabolism. HisFH is responsible for the HisH-catalyzed hydrolysis of glutamine to glutamate and ammonia, which is then used for a cy
the recombinant SlGAME36 enzyme did not exhibit acyltransferase activity with any of the core SGA substrates (e.g.,α-tomatine) (Fig.2c,dand, Supplementary Figs.5and6; in dark purple) suggesting its strict substrate preference towards hydroxylated SGAs. Through a sequence similarity search, we...