For scRNA-seq, data from female microglia were analyzed by using RunPCA with VariableFeatures, and the top 20 principal components (PCs) of the datasets were used for clustering and tSNE/uniform manifold approximation and projection (UMAP) visualization. For microglial transcriptome analysis, clusters...
Guided by gut sensory cues, humans and animals prefer nutritive sugars over non-caloric sweeteners, but how the gut steers such preferences remains unknown. In the intestine, neuropod cells synapse with vagal neurons to convey sugar stimuli to the brain
Cells were imaged on Nikon W1 spinning disc confocal microscope (Center for Advanced Microscopy, Northwestern University), 1 frame per 10 s at 100× magnification. Immunohistochemistry Mice were anaesthetized with an intraperitoneal injection of 240 mg kg−1 Avertin and transcardially perfused ...
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Danelljan, M., Häger, G., Khan, F.S., Felsberg, M.: Accurate scale estimation for robust visual tracking. In: Proceedings of the British Machine Vision Conference BMVC (2014) Google Scholar Danelljan, M., Häger, G., Khan, F.S., Felsberg, M.: Learning spatially regularized corr...
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Integration of spatial and single-cell transcriptomics localizes epithelial cell-immune cross-talk in kidney injury. JCI Insight. 2021;22:e147703. Article Google Scholar Staruschenko A. Regulation of transport in the connecting tubule and cortical collecting duct. Compr Physiol. 2012;2:1541–84....
Pancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve and 20 treated) using single-cell/nucleus RNA sequencing, bulk-proteogenomics, sp
(16 samples, 65,775 cells).b117 patient samples and 377,614 cells were included for subsequent analysis. The Uniform Manifold approximation and Projection (UMAP) plot showing the cell distribution.cThe UMAP plot showing the major cell populations.dThe UMAP plot displaying tumor cell clusters. 20...
. Nanobioengineering is amongst the most challenging domain of bionanotechnology that is advancing rapidly. Nanobioengineering is an applied science that integrates the fundamentals of basic sciences like physics, chemistry, and biology to engineer material properties in nano-dimensions for manifold bio-...