Total genomic DNA was extracted using the PURRGENE® DNA Isolation Kit (Gentra Systems Inc., Minneapolis, MN USA), and total RNA was extracted using RNeasy® Mini Kit (Qiagen, Valencia, CA USA). Based on the syntenic relationship among black muntjac, Indian muntjac, and cow and ...
The levels of MMP-2, collagen II, and aggrecan in the NP cell culture medium were quantified using Quantikine ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. Small interfering RNA and circ-FAM169A overexpression plasmid construction According to the circRNA...
Department of Microbiology, University of Minnesota, Box 196, Twin Cities, Minneapolis, MN 55455-0312, USA Martin Dworkin Professor Dr. (Editor-in-Chief) (Editor-in-Chief) Department of Microbiology and Immunology, Stanford University Medical School, 299 Campus Drive, Fairchild, D039, Stanford, ...
We first used a threading method to simulate a model of the MOR–CCKBR complex27. Subsequently, a computational prediction was performed to analyze the interface of the MOR–CCKBR interaction. The prediction results showed that the intracellular loop 2 (IL2), extracellular loop 2 (EL2), transmem...
(Woburn, MA) for TNF-α and IL-8 and from R&D Systems (Minneapolis, MN) for IL-1β, IL-12p40, using 96-well microtiter plates, according to each manufacturer's instructions. The levels of TNF-α, IL-8, and IL-12p40 were calculated by using a standard curve obtained with rhIL-1...
that matched the supporting connector attached to the implant, in addition to a 36-pin Omnetics connector (A79029-001, Omnetics Connector Corporation, Minneapolis, MN, USA) for the electrical signals from the electrodes. The total weight of all elements was approximately 16 g. The load was ...
[IEEE 2006 American Control Conference - Minneapolis, MN, USA (2006.06.14-2006.06.16)] 2006 American Control Conference - Discrete implementation and gener... R Miklosovic,A Radke,Z Gao 被引量: 0发表: 2006年 A NetConf Network Management Suite: ENSUITE Context ...ow a more and more ...
(Invitrogen, 1% fetal bovine serum (Hyclone, Logan, UT)] and growth factor components [103 U/ml LIF, 10 ng/ml recombinant human basic FGF (Sigma Aldrich, 20 ng/ml recombinant mouse EGF, 15 ng/ml recombinant rat glial cell derived neurotrophic factor (GDNF; (R&D Systems, Minneapolis, MN...
(Minneapolis, MN, USA), respectively, and dissolved as the manufacture protocol. 2.2. Expression Vector Constructs and Stable Transfection The expression vector of human GDF15 (pcDNA3-GDF15) was constructed and transfected into bladder carcinoma T24 cells by electroporation and selecting clonally with...
Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN, USA Jian-Ping Wang Chemistry and Nanoscience Center, National Renewable Energy Laboratory, Golden, CO, USA Matthew C. Beard Department of Physics, University at Buffalo, State University of New York, Buffalo,...