The “on” or “off” switch for operon expression is an important adaptation as it allows cells to respond to environmental changes actively and efficiently. The first described operon was the lactose operon in Escherichia coli, also commonly referred to as the lac operon [3]. How does the ...
These oddities can more easily be explained, if other galactosides were the natural substrates to which the lac-operon had been adapted first. Galactosylglycerols, which are readily obtained from galactolipids (widespread in most plant materials), are appropriate candidates both in terms of ...
2b). No consensus sequence emerged from the sequence analysis, which can be explained by the much higher theoretical diversity of the designed library as compared to the actual diversity that can be handled with the phage display method. Clones characterization Specificity of the isolated clones was...
This could be explained by the fact that many proteins of the divisome are essential and corresponding Tn mutants could not be evaluated. These results could also reflect the possibility that Rod complex proteins are able to act within the divisome63, while divisome complex proteins cannot act in...
Using lac-operon fusions to DNA damage inducible (din) loci we investigated whether these observations could be explained by an inability of topA strains to efficiently induce DNA damage responses. Mitomycin C (MMC)-induced expression of lac-operon fusions to uvrB and to a second SOS locus, ...
The presence of persisters explained the biofilm phenotype, and with it, the most important property of chronic infections in general. A common feature of chronic infections is the ability of a pathogen to hide from the immune system. Biofilm exopolymers protect cells from the large components of...
In addition to serC, the purine operon, and lmo0517 (pgm), the more extensive EMA also suggests aroB to be critical for the intracellular survival. In addition to the result of EPA, glpD revealed to be less important for the system. This discrepancy might be explained by considering ...
We reasoned that the addition of lac operators on the chromosome in the genome-integrated expression systems might influence the endogenous lac operon activity. Therefore, we also measured the LacI levels in selected strains. The production clones were compared in micro-titer fermentations, under fed...
The thermoinduced expression system has been improved throughout the years (Table1). One of the earliest remarkable advances was the overproduction of the cI repressor by construction of plasmids bearing the lac operon promoter adjacent to the cI repressor gene [56]. Latter on, the first expressi...
The production of 3-methyl-1-butanol can be explained by a withdrawal of 2-keto-isocaproate, the precursor of leucine biosynthesis, which is first decarboxylated and then reduced by host intrinsic enzymes to the corresponding alcohol [33]. Table 1 Influence of yeast extract on product titers ...