These oddities can more easily be explained, if other galactosides were the natural substrates to which the lac-operon had been adapted first. Galactosylglycerols, which are readily obtained from galactolipids (widespread in most plant materials), are appropriate candidates both in terms of ...
For that, strains and single-cells were screened by sequencing the ITS1-5.8S-ITS2 region of the ribosomal operon, as explained in Blanquart et al.9. Then, Illumina whole-genome sequencing was performed on a selection of 50 cultivated strains (where the flow cytometry-estimated bacterial ...
2b). No consensus sequence emerged from the sequence analysis, which can be explained by the much higher theoretical diversity of the designed library as compared to the actual diversity that can be handled with the phage display method. Clones characterization Specificity of the isolated clones was...
We reasoned that the addition of lac operators on the chromosome in the genome-integrated expression systems might influence the endogenous lac operon activity. Therefore, we also measured the LacI levels in selected strains. The production clones were compared in micro-titer fermentations, under fed...
These mass peaks can be explained to be composed of LAhexa + Kdo2 + Hep2 + Hex1 and LAhexa + Kdo2 + Hep2 + Hex1 + P-EtN1 + l-Ara4N1, respectively. Further mass peaks at 3,199.6 and 3,361.7 Da correspond to the addition of one and two Hex residues, respectively (Fig. 8D...
The presence of persisters explained the biofilm phenotype, and with it, the most important property of chronic infections in general. A common feature of chronic infections is the ability of a pathogen to hide from the immune system. Biofilm exopolymers protect cells from the large components of...
This may be explained by the observation that expressing lacI is beneficial for E. coli strains growing on glucose because it represses expression of the lac operon. Stoebel et al. [46] have in fact found that cells with the genomic copy of lacI show some residual lacA activity when grown...
The design of synthetic genetic circuits typically overlooks contextual features by considering that phenotypic performance can be explained by the DNA sequence of the synthetic construct alone. However, this over-simplification has negative implications; for example, it requires considerable effort to adapt...
Diffusion is an example of an emergent process because the mechanism is explained by the collective interaction of the dye and water molecules rather than any single or aggregate components. She observed that students’ misconceptions of emergent processes are robust because they misinterpret emergent ...
The thermoinduced expression system has been improved throughout the years (Table1). One of the earliest remarkable advances was the overproduction of the cI repressor by construction of plasmids bearing the lac operon promoter adjacent to the cI repressor gene [56]. Latter on, the first expressi...