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Rat anti-human α6 integrin mAb GoH3 was from PharMingen (San Diego, CA), mouse anti-human β1 mAb 2253 from Chemicon (Temecula, CA), and mouse anti-human Myc mAb 9E10 and anti-phosphotyrosine mAb from Calbiochem. Human placental and murine EHS laminins as well as all other reagents ...
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The slides were washed in TBS and incubated in mouse anti-serotonin transporter (SERT) (Chemicon, Temecula, CA, FPS USA) in 10% goat serum overnight. The following day, the slides The FPS protocol was conducted as previously described.20,21 On days 1 and 2, the rats were acclimated to...
Sections were then incubated with primary antibody against gp91phox at a dilution of 1:100;34 3-nitrotyrosine at a dilution of 1:100 (Upstate Biotech, Lake Placid, NY); or anti-RelA (Chemicon, Temecula, CA) at a dilution of 1:100 in PBS containing 1% bovine serum albumin for 60 ...
(47-mm diameter; Durapore Membrane filters, Millipore Sigma, Temecula, CA, USA) was used to filter the 1L sample into an acid-washed, 1L flask to remove large aggregates from the sample. The resulting filtrate is then immediately vacuum filtered through an acid-washed, sterilized glass funnel...
A commercially available version of the Columbia bubble CPAP setup (BubbleFlow; Fisher & Paykel, Auckland, New Zealand) set to deliver 5 cm H2O was applied to size 2 nasal CPAP prongs (Hudson RCI, Temecula, CA) and connected to the in vitro lung model (Fig. 1). Studies were performed ...
LDHA inhibitors, namely FX11 (427,218, EMD Millipore, Temecula, CA, USA) and oxamate (A16532.06, Thermo Fisher Scientific, Heysham, UK), were administered intrathecally using a PE5 catheter. FX11 and oxamate were dissolved in a solution containing 30% (v/v) dimethylsulfoxide (DMSO) (D...
(S0553, Epitomics, Burlingame, CA, USA), anti-CytC (3895-1, Epitomics), anti-Flag (F3165, Sigma-Aldrich), anti- glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, CHEMICON, Temecula, CA, USA), anti-GFP (sc-9996, Santa Cruz Biotechnology), anti-GSK- 3b (NBP1-47470, Novus, ...