Leading EdgeReviewThe Human Transcription FactorsSamuel A. Lambert, 1,9 Arttu Jolma, 2,9 Laura F. Campitelli, 1,9 Pratyush K. Das, 3 Yimeng Yin, 4 Mihai Albu, 2 Xiaoting Chen, 5Jussi Taipale, 3,4,6, * Timothy R. Hughes, 1,2, * and Matthew T. Weirauch 5,7,8, *1 Department...
transcriptionhumantfsfactorsbindingcincinnati LeadingEdgeReviewTheHumanTranscriptionFactorsSamuelA.Lambert,1,9ArttuJolma,2,9LauraF.Campitelli,1,9PratyushK.Das,3YimengYin,4MihaiAlbu,2XiaotingChen,5JussiTaipale,3,4,6,*TimothyR.Hughes,1,2,*andMatthewT.Weirauch5,7,8,*1DepartmentofMolecularGenetics,Universi...
However, it has been challenging to identify the cell types associated with AMD given the genetic complexity of the disease. Here we perform massively parallel single-cell RNA sequencing (scRNA-seq) of human retinas using two independent platforms, and report the first single-cell transcriptomic ...
Stem cells are maintained by transcriptional programs that promote self-renewal and repress differentiation. Here, we found that the transcription factor c-Myb was essential for generating and maintaining stem cells in the CD8+ T cell memory compartment.
In brief, coexpression modules were inferred by the “runGENIE3” function, and potential direct binding targets (regulons) of transcription factors were identified using the human motif database of 10 kb around the transcription start site (TSS) for RcisTarget. Then, regulon activity in cell ...
3a-b). To identify human TET cells, we performed inferCNV analysis. Clusters 3 and 0 were utilized as reference normal groups, representing the fibroblasts (Fig. 3c). Four clusters were identified as the epithelial cell population. Subsequently, we isolated all the cancer cells and conducted an...
Here, we profiled the spatiotemporal gene programs of the human embryonic kidneys at 9 and 18 post-conception weeks (PCW) by integrating the application of microarray-based spatial transcriptomics and single-cell transcriptomics. Results We mapped transcriptomic signatures of scRNA-seq cell types upon...
Microbiome and tryptophan metabolomics analysis in adolescent depression: roles of the gut microbiota in the regulation of tryptophan-derived neurotransmitters and behaviors in human and mice Manfei Zhou, Yichun Fan, Liuting Xu, Zheng Yu, Sizhe Wang, Huaisha Xu, Jiuping Zhang, Linwei ...
We used each method to acquire nuclear transcriptomic and DNA accessibility maps for >60,000 single cells from human adult visual cortex, frontal cortex, and cerebellum. Integration of these data revealed regulatory elements and transcription factors that underlie cell-type distinctions, providing a ...
However, the yellow fever vaccine induced an early transient signature of T and B cell activation at day 1, followed by delayed antiviral/interferon and plasmablast signatures that peaked at days 7 and 14–21, respectively. Thus, there was no evidence for a ‘universal signature’ that ...