SptF PCR products were purified and ligated into the NdeI/HindIII-digested pET-22b (+) vector, using an In-Fusion® HD Cloning Kit (TaKaRa Clontech). The identities of the resulting plasmids were confirmed by DNA sequencing. Production and purification of SptF and its mutants In this ...
The authors also drew the conclusions that making an animation helps children to express what they had learned, since actions and thoughts could be expressed in a visual product, which is then a way to make the children’s ideas and theories visible also to others. In this study, the ...
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Monitoring and mapping of changes in land cover and land use is essential to the sustainable development and management of the area. The study attempts to track changes in LULC pattern for the years 2002, 2013, and 2023 in the Hindon River Basin, a major tributary of the Yamuna River, ...
First, we chose to build tissue recombinants by admixing PSC27 cells with PC3 cells, which are a typical prostate cancer cell line of high malignancy, at a pre-optimized ratio (1:4)14. The cells were then subcutaneously implanted into the hind flank of mice with non-obese diabetes and ...
Brainy Is the New Sexy —— "The Physicist Bechind Thc Big Bang Theory's Ncrd Cred" 来自 Semantic Scholar 喜欢 0 阅读量: 153 作者: 李晓涵 摘要: 与《老友记》中贴近日常生活的普通青年不同,《生活大爆炸》的主角是一群智商超群,情商堪忧的理工宅男,讲着常人难懂的科学术语,有着异于常人的古怪思维...
For at forhindre legemesbeskadigelse ved montering eller service af denne enhed i et rack, skal du sikre at systemet står stabilt. Følgende retningslinjer er også for din sikkerheds skyld: •Enheden skal monteres i bunden af dit rack, hvis det er den eneste enhed i racket....
SOFTWAREN KAN INDEHOLDE PRODUKTAKTIVERING SAMT ANDEN TEKNOLOGI BEREGNET TIL AT FORHINDRE UAUTORISERET KOPIERING. AKTIVERINGSTEKNOLOGIEN FORHINDRER DIG I AT BRUGE SOFTWAREN, SÅFREMT DU IKKE FØLGER AKTIVERINGSPROCEDUREN BESKREVET I SOFTWAREN OG DOKUMENTATIONEN. Besøg http://www.adobe....
Why it's awesome: Sun bears often stand on their hind legs and can look so similar to humans they've been mistaken for people wearing costumes. Sun bears are the smallest bears in the world. While grizzly bears (Ursus arctos horribilis) can weigh more than 700 pounds (320 kilograms), su...
2d) was also constructed where the HolA-Cs was changed to RzmA-Cs domain, except the p15A-cm-holA was linearized by digestion of HindIII and EcoRV. The swapping of Cs subdomain (N-lobe of Cs domain) followed the same procedure as the full-length Cs swapping method by using fragments ...