Hercules (1997) IMDB *** Some funny scenes, with characters mistating quantities being even or odd. Hidden Figures (2016) *** IMDB Not the masterpiece people are declaring, but a polished and reasonably accurate movie about the black women "computers", in particular Katherine Johnson, who wo...
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Present address: Bio-Rad Laboratories, Hercules, CA, USA These authors contributed equally: Chak Ming Leung, Pim de Haan. Authors and Affiliations Integrative Sciences and Engineering Programme, National University of Singapore, Singapore, Singapore Chak Ming Leung Pharmaceutical Analysis Group, Groningen...
To test our hypothesis that nonfunctional peroxisomes were directly caused by the lack of AlLUC1 protein, we applied RNAi to theAlLuc1gene. This produced a non-luminescent phenotype (Fig.4Dand Supplementary Movie2). Verification by real-time qPCR confirmed that the expression level ofAlLuc1was ...
parvum) was performed using the Anyplex™ II STI-7 Detection Kit (Seegene, Seoul, Korea), in accordance with the manufacturer's protocol, in a CFX96 real-time thermocycler (Bio-Rad, Hercules, CA, USA). Each PCR was performed in 5 μl of extracted DNA, 4x STI-7 TOM, and Anyplex...
Protein bands were detected using the Bio-Rad ChemiDoc™ (Hercules, CA, USA). GAPDH was used as an internal control. Cytokine assay Spleen cells were activated with plate-bound anti-CD3 (5 µg/ml) plus soluble anti-CD28 (2 µg/ml, BD Biosci- ence) and stimulated with ...
Labor like Hercules —William H. Gass The only time some people work like a horse is when the boss rides them —Gabriel Heatter Toiled like movers trying to get a refrigerator into a fifth-floor walk-up —Russell Baker Toiling like a bee in a hive —Noël Coward, lyrics for “World ...
In the experiment, a final concentration of 20 nM scaffold, 200 nM of each staple strand,5 mM Tris, 5 mM NaCl, 1 mM EDTA, and 18 mM MgCl2, at pH 8.0 in aqueous solutions were made and then subjected to thermal annealing in a thermal cycler (Bio-Rad, Hercules, CA) for self-...
The persistent cereal endosperm constitutes the majority of the grain volume. Dissecting the gene regulatory network underlying cereal endosperm development will facilitate yield and quality improvement of cereal crops. Here, we use single-cell transcrip