ProteinsTo characterize endogenous molecules and activities of the Golgi complex, proteins in transit were >99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment. The loss of proteins in transit resulted in condensation of the Golgi cisternae and stacks. Isolation of a stacked ...
In eukaryotes, the retromer complex plays a crucial role in the sorting and retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN). Despite its importance, the molecular details of this intracellular transport process remain unclear. Here, we have identified a Golgi...
COG subunits, golgins, and Rabs were expressed as Gal4 binding or activation domain fusion proteins from pGAD/pGBD/pGBDU plamids (66). Rab binding domain fusion proteins contained mutations to lock the protein in the active (T) or inactive (D) conformation (67). Pairs of fusion constructs...
Mitochondria and the Golgi Complex*Author links open overlay panelGeoffrey H. Bourne, Hrishi Bhu TewariShow more Add to Mendeley Share Cite https://doi.org/10.1016/B978-0-12-119254-9.50016-XGet rights and contentAccess through your organization Check access to the full text by signing in ...
After the exit of STING from the Golgi/TGN, STING translocates to recycling endosomes26,37. In line with this, colocalizations of mEos4b-STING with recycling endosomal proteins (transferrin receptor (TfnR) and Rab11) increased only 120-360 min after DMXAA stimulation (Fig. 3a−f). ...
Sec14 proteins are involved in the cytosolic transport of secretory proteins from the Golgi apparatus in yeast [30]. More recent research [31] indicates that Sec14 proteins are essential in membrane trafficking, connecting lipid metabolism with phosphoinositide signaling through transport of phosphatidylin...
(Adobe Systems). For immunohistochemistry on presynaptic proteins, brain slices were acquired using a ×63 oil-immersion objective (N.A. 1.4). Fifty µm-thickZ-stacks were acquired with a step size of 0.8 µm in a 245 × 245 µm square (1024 × 1024 resolution) ...
ER and Golgi (Creemers2002; Gidalevitz et al.2013). Characteristic features of secreted proteins, such as biological activity, half-life time, or affinity to specific intracellular binding partners are modified by these events. The best-known example for a precursor protein (pro-protein) that un...
The GA receives proteins from the endoplasmic reticulum (ER) and modifies them through glycosylation, phosphorylation, and proteolytic cleavage. The GA also acts as a sorting center, packaging proteins into vesicles for transport to different cellular compartments, and ensures quality control by ...
and assembly of new viral nucleocapsids from genomic RNA and N proteins occurs in the cytoplasm, whereas new particles are produced by the synergistic action of both the endoplasmic reticulum and the Golgi compartments [14]. Lastly, assembly of the genomic RNA and structural proteins into new vir...