Cellular biology Protein -protein interactions at the Golgi complex and their roles in apoptosis and mitosis THE JOHNS HOPKINS UNIVERSITY Carolyn E. Machamer SbodioJuan IgnacioGolgin-160, ubiquitously expressed in vertebrates, localizes to the cytoplasmic side of the Golgi complex and has a large C...
Inside the body, the interactions between drugs and various types of proteins (like enzymes) activate drugs for their action [305]. However, cancer cells exhibit resistance to drugs by altering a catalytic site or structure of an enzyme which affects their interaction with drugs and thus culminate...
In nerve cells it occurs as a reticulum of wide meshes around the nucleus. The size of Golgi complex is also variable. It is small in muscle cells but quite large in the nerve and gland cells. Generally the complex is well developed in an active cell while in old cells the complex dimi...
COG subunits, golgins, and Rabs were expressed as Gal4 binding or activation domain fusion proteins from pGAD/pGBD/pGBDU plamids (66). Rab binding domain fusion proteins contained mutations to lock the protein in the active (T) or inactive (D) conformation (67). Pairs of fusion constructs...
Sec14 proteins are involved in the cytosolic transport of secretory proteins from the Golgi apparatus in yeast [30]. More recent research [31] indicates that Sec14 proteins are essential in membrane trafficking, connecting lipid metabolism with phosphoinositide signaling through transport of phosphatidylin...
ER and Golgi (Creemers2002; Gidalevitz et al.2013). Characteristic features of secreted proteins, such as biological activity, half-life time, or affinity to specific intracellular binding partners are modified by these events. The best-known example for a precursor protein (pro-protein) that un...
After the exit of STING from the Golgi/TGN, STING translocates to recycling endosomes26,37. In line with this, colocalizations of mEos4b-STING with recycling endosomal proteins (transferrin receptor (TfnR) and Rab11) increased only 120-360 min after DMXAA stimulation (Fig. 3a−f). ...
Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular... Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only ...
(Adobe Systems). For immunohistochemistry on presynaptic proteins, brain slices were acquired using a ×63 oil-immersion objective (N.A. 1.4). Fifty µm-thickZ-stacks were acquired with a step size of 0.8 µm in a 245 × 245 µm square (1024 × 1024 resolution) ...
For this purpose, an intricate export machinery emerged that is predominantly governed by the transport and Golgi organization (TANGO) 1 protein family; these are transmembrane proteins located at the endoplasmic reticulum exit sites (ERES) found in most metazoans2,3. The simultaneous emergence of ...