Enzymatic breakdown of sphingomyelin by sphingomyelinase (SMase) is the main source of the membrane lipids, ceramides, which are involved in many cellular physiological processes. However, the full-length structure of human neutral SMase has not been res
Preparation of libraries for DNA sequencing for Illumina systems involves multiple steps. In a general workflow, purified DNA is fragmented, end-repaired, and A-tailed; adapters are ligated to the DNA fragments; libraries are...
State-of-the-art super-resolution microscopy allows researchers to spatially resolve single proteins in dense clusters. However, accurate quantification of protein organization and stoichiometries requires a general method to evaluate absolute binder labeling efficiency, which is currently unavailable. Here ...
The sequence alignment and characterization of the deduced proteins were carried out using different tools available in SDSC Biology Workbench (http://seqtool.sdsc.edu). Multiple sequence alignment was done using ClustalW pairwise alignment algorithm. To find out general features of the cDNA sequence...
In general, non-coding perturbations did not induce widespread changes in expression. Almost half of all perturbations (47%) resulted in a single DEG within 1 Mb of the target site, and an additional 32% resulted in fewer than five DEGs (Fig. 4B). These results suggest that CRISPRi ...
The measurement of enzyme activity was calculated as the percentage uncleaved RNA substrate (P1) compared to the input RNA (untreated P1). Data (mean± SE) were analyzed by general linear model analysis of variance (ANOVA), with subsequent comparison between means using a post hoc Tukey test....
Quantitative western blotting requires careful interpretation, particularly around non-uniformities. Variability in western blotting occurs most often with unequal protein concentrations, inconsistent sample loading onto the gel, and irregularities during transfer – all normal, human realities of the process....
Additionally, there is a high risk of cancer recurrence, and systemic therapy has limited effectiveness [1, 2]. Therefore, the quest for novel biomarkers pertaining to CCA-targeted molecular therapy is a pivotal task in tumor research. The precise regulation of transcription plays a pivotal role ...
Loew, O. A new enzyme of general occurrence in organismis. Science 11, 701–702 (1900). The discovery of catalase, the first known scavenging enzyme. Article CAS PubMed Google Scholar McCord, J. & Fridovich, I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein)....
In general, non-epithelial cells express NHEs (mostly the ubiquitous NHE1), AEs (especially AE3), and NBCs. In epithelial cells, the above-mentioned transporters exhibit distinct membrane domain locations, and some transporters show exclusive expression in the apical membrane or basolateral membranes...