In lung cancer cells, circNBPF10 negatively regulated the expression of miR-224, whereas miR-224 directly targeted the expression of PBX3. The results of the rescue experiment confirmed that PBX3 was the key gene for the promoting effect of circNBPF10 on the malignant progression of lung ...
29 Therefore, it is conceivable that the enforced expression of miR-29b in cancer cells may interfere with multiple oncogenic mechanisms, including tumor-suppressor promoter methylation,34 extracellular matrix remodeling35 and anti-apoptotic signaling.31 Moreover, it is of note that miR-29b/miR-29a ...
qPCR was used to detect the relative expression of M2 macrophage markers Arg-1, CCL-18 and IL-10. RNA extraction and qPCR Total RNAs were extracted from cells or tumor tissue and paracancer tissue of patients and mice by using RNAiso Plus (Cwbio, China), followed by cDNA synthesis with ...
Most patients with prostate cancer inevitably progress to castration-resistant prostate cancer (CRPC), at which stage chemotherapeutics like docetaxel become the first-line treatment. However, chemotherapy resistance typically develops after an initial p
Fig. 3: FOXJ2 expression was elevated by ARHGAP9 overexpression. a RNA-sequencing data showed that ARHGAP9 overexpression significantly induced mRNA level of FOXJ2 in HepG2 cells. **P < 0.01. b HepG2 and MHCC-97H cells were overexpressed with ARHGAP9. The protein level of FOXJ2 was...
cZNF215 was overexpressed in HuCCT1 cells by lentiviral transfection and knocked down through short hairpin RNA (shRNA) in RBE cells, which were labeled with firefly luciferase allowing for tracking by the in vivo imaging system (IVIS). As a result, tumor growth was markedly promoted by cZN...
(B) Western blotting showed that only HOK cells transfected with pEGFP-N3-YOD1 had a band at 65 kD, while the other groups had no bands. These results showed that YOD1 was successfully overexpressed in HOK cells after transfection with pEGFP-N3-YOD1. DownLoad: Full-Size Img PowerPoint ...
CircNFATC3 was significantly overexpressed in GC tissues and was positively associated with tumor volume. Functionally, the proliferation of GC cells decreased significantly after circNFATC3 knockdown in vivo and in vitro. Mechanistically, circNFATC3 bound to IGF2BP3 in the cytoplasm, which ...
First, the expression of these lncRNAs was overexpressed in tumor tissue than normal tissue with P-value < 0.001 (Fig. 8A, B); Second, these lncRNAs were significantly associated with prognosis (Fig. 8C, D); Third, based on the ceRNA hypothesis, these lncRNAs should be a negative ...
Moreover, YAP increased the iron concentration in HCC cells through transcriptional elevation of TFRC via its O-GlcNAcylation. With YAP knockdown or YAP-T241 mutation, the increased sensitivity to ferroptosis induced by O-GlcNAcylation was abolished. In addition, the xenograft assay confirmed that O...