Figure 1. Total protein normalization using the No-Stain Protein Labeling Reagent:Bolt 4-12% Bis-Tris Plus gels were loaded with HeLa Lysate ranging from 10 to 50 µg and electrophoresed using MES running buffer. Proteins from the gels were transferred onto PVDF membranes using the Invitrogen...
we divided viable mouse distal colon tissues from conventionally raised mice fed a WSD into two pieces and stimulated the first piece ex vivo with a buffer control, while the second piece from the same mouse was stimulated with propionate. Confirming...
DPI, diphenyleneiodonium, an inhibitor of NADPH oxidase. Each data point is shown as an open circle. Data are shown as mean ± standard deviations, n = 3 technical replicates. The experiment was performed independently twice with similar results. d, The activity of the NOX2 mutant ...
depending on the source site were mixed 1:1 with 4 mM H2SO4, homogenized by shaking at room temperature for 1 h, and centrifuged at 12,000 × gfor 10 min. The supernatant was collected with a
Dawson HD: Comparative assessment of the pig, mouse, and human genomes; A structural and functional analysis of genes involved in immunity. The Minipig in Biomedical Research P A McAnulty. Edited by: Dayan A, Hastings KH, Ganderup N-C. 2011, Boca Raton, FL: CRC Press (Taylor & Francis...
High DPI awareness. By default the game will not run in high DPI mode, but it will be aware of high DPI contexts. This prevents things from looking fuzzy with weird scaling settings. Automated GitHub and GitLab packaging pipelines.
pulex as we found this domain in insects (e.g. Tribolium castaneum, Nasonia vitripennis and Drosophila melanogaster) and vertebrates (e.g. Mus musculus) (data not shown). Another important difference observed among InR is the absence of the furin-like cystein-rich domain in Dappu_InR-2. ...
Matthias K, Kirchhoff F, Seifert G, Huttmann K, Matyash M, Kettenmann H, Steinhauser C: Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus. J Neurosci. 2003, 23: 1750-1758. CAS PubMed Google Scholar ...
Goat anti-mouse IgG (H + L) Secondary Antibody, HRP ABclonal Technology Cat# AS003; RRID: AB_2769851 Bacterial and virus strains Escherichia coli strain DH5a Yang lab stock N/A Agrobacterium tumefaciens strain GV3101 Yang lab stock N/A Escherichia coli strain BL21 (DE3) Yang lab stock N/...
Human coronaviruses can replicate in mice but do so poorly and do not cause disease due to the specificity of the viral spike fusion proteins and differences between the human and mouse orthologs of the primary SARS-CoV and CoV-2 receptor (ACE2). To circumvent this we used two different mo...