A metod is described for obtaining a sample-stacking effect before sodium dodecyl sulfate-agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide-agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be ...
Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) SDS-PAGE was performed following the method of He et al. (2014). The protein powder (2 mg) was dissolved in 0.5 mL of sample buffer (0.08 mol L–1 Tris-HCl buffer, pH 6.8), 1% (w/v) SDS, 2% (v/v) 2-β-merca...
aPotential molecular pathomechanisms of FXTAS.bEMSA of the interaction of rCGG20with ASO–CCGs having 9 and 11 LNA residues. The graph presents means with SDs.N = 3 independent samples for each concentration. Kd, a dissociation constant [nM]. Light blue/dots, 9 nt; dark blue/square...
Samples were boiled for 10 min, centrifuged for 5 min at 35,000 × g and 20 µl aliquots (=20 μg total protein) were separated on a 10% SDS-PAGE gel containing 0.5% (v/v) trichloroethanol. Following electrophoreses, proteins were UV cross-linked for 5 min and ...
Volume was calculated using the formula L× W2× 0.52, where L is the longest dimension and W is the perpendicular dimension. Differences in survival were determined for each group by the Kaplan–Meier method and the overall P value was calculated by the log-rank test using the survival R ...
A A-PAGE electrophoretic patterns of gliadins of CS and DS6E(6D). B 15% SDS-PAGE electrophoretic patterns of glutenins from CS, DS1E(1D). C-D Rheological properties of CS, DS1E(1D) respectively Full size image HWM-GS accounts for 16% percent of the total wheat gluten protein, but ...
Protein lysates were then resolved in SDS-PAGE (Beyotime, China) gel and transferred to polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membranes were probed with primary antibodies: PI3K, p-PI3K, Akt, p-Akt (Cell Signaling Technology, USA), and β-actin (Santa Cruz, ...
The final step involves analyzing the translation products on an SDS-PAGE gel to determine their length. The gel is normally treated with a fluorographic-enhancing reagent to reduce the exposure time to X-ray film. Truncated proteins are identified by size differences when compared to full-length...
To assess the formation of dimers, the samples were subjected to SDS-PAGE under reducing conditions, followed by transfer to nitrocellulose and western blotting with a rabbit anti-RNAse A antibody conjugated to HRP. Circles=RNAse A alone; Squares=RNAse A after incubation with cigarette smoke; ...
A process for preparing silicon carbide by carbothermal reduction is disclosed. This process involves rapidly heating a particulate reactive mixture of a silica source and a carbon source to form a pr