Molecular docking is a computational technique that predicts the binding affinity of ligands to receptor proteins. Although it has potential uses in nutraceutical research, it has developed into a formidable tool for drug development. Bioactive substance
The basic protocol for performing an IP is diagrammed below, where the order (sequence) of steps can be done in two different ways. Diagram of immunoprecipitation (IP) using either pre-immobilized or free antibodies. Each step invo...
The main enriched KEGG pathways among the DEGs on day 1 of the S treatment were “Transcription factors,”“Plant hormone signal transduction,”“Photosynthesis proteins,”“Photosynthesis – antenna proteins,” and “MAPK signaling pathway – plant.” Notably, the DEGs associated with the plant ...
Gene expression involves transcription, translation and the turnover of mRNAs and proteins. The degree to which protein abundances scale with mRNA levels and the implications in cases where this dependency breaks down remain an intensely debated topic. Here we review recent mRNA–protein correlation st...
Tolerance of low temperature has a significant impact on survival and expansion of invasive snail Pomacea canalicuata. Cold acclimation can enhance cold tolerance of Pomacea canalicuata. To elucidate the molecular mechanism of P. canaliculata’s response
When growing on glucose, it is expressed during the stationary phase, although its regulation is complex, since it involves several transcription factors, two sigma factors and two promoters [7]. The gene coding this enzyme, acs, is within an operon, together with another two genes: yjcH, ...
Other studies have provided evidence that the control of late megakaryocytic differentiation by TGF-β1 could involve the transcription factors c-jun and c-fos as downstream effectors.243, 244 TGF-β and the development of dendritic antigen-presenting cells To discuss the effects of TGF-β on ...
Construction of single-cell transcription landscape of cotton hypocotyl during dedifferentiation Hypocotyl is the main explant material used for Agrobacterium-mediated genetic transformation of cotton and has no branch structure (Fig. 1a, b). Previously, we found that Agrobacterium mainly expresses the tra...
The latter involves a two-step recombination/transposition procedure to effectively remove one of the piggyBac ends26,27. Results tTAo driven by the Lsbnk promoter is expressed in early embryos and other developmental stages The DR2 driver construct contains the Lsbnk promoter upstream of the t...
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