Numerous studies have used human pluripotent stem cell-derived cerebral organoids to elucidate the mystery of human brain development and model neurological diseases in vitro, but the potential for grafted organoid-based therapy in vivo remains unknown. Here, we optimized a culturing protocol capable ...
These models, generated using human embryonic stem cells or induced pluripotent stem cells, are called "organoids". Organoids have successfully and rapidly uncovered new mechanisms of human brain development in health and disease. In the future, we envision that this strategy will enable faster and...
2b). Overall, the resulting hHOs contained significant myocardial tissue with epicardial tissue clustered near the outer surface of the organoids, mimicking the anatomical structure of the developing embryonic heart (Fig. 1i). Transcriptomic analysis reveals hHOs closely model human fetal cardiac ...
Finally, we explained graft efficacy of our human embryonic stem-cell-derived epicardium model by noting its similarity to human fetal epicardium. Overall, our study defines epicardial programs of regenerative angiogenesis absent in adult hearts, contextualizes animal studies and defines epicardial states...
(FST) has been demonstrated to enhance the viability, proliferation, and wound healing of primary cultured embryonic trophoblast cells [255]. This process has been further corroborated in the microfluidic device, revealing that FST acts as a chemokine to promote JNK signaling pathway-driven ...
(PRMTs), suggesting OXR1-dependent mechanisms regulating gene expression during neurodevelopment. We model the function of OXR1 in early human brain development using patient-derived brain organoids revealing that OXR1 contributes to the spatial–temporal regulation of histone arginine methylation in ...
Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression © 2024 Main Germ cells give rise to totipotency and ensure heredity and evolution. Human PGCs are thought to be specified at around embryonic day 12 (E12) to E16 (2 weeks post-fe...
Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases Grassi et al. Cell Death Dis. 2019; 10(3):1–5. Lung Modeling of fibrotic lung disease using 3D organoids derived from human pluripotent stem cells Strikoudis et al. Cell Rep. 2019...
A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147 (1998). CAS PubMed Google Scholar Martin, G. R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl Acad. Sci. ...
human embryos and human pluripotent stem cells, we show that the reorganization of the embryonic lineage is mediated by cellular polarization leading to cavity formation. Together, our results indicate that the critical remodelling events at this stage of human development are embryo-autonomous, ...