However, the clinical utility of EVs has thus far been limited by the fact that most EV isolation methods are tedious, nonstandardized, and require bulky instrumentation such as ultracentrifugation (UC). Here, we report a size-based EV isolation tool called ExoTIC (exosome total isolation chip)...
The exosome total isolation chip. ACS Nano 11, 10712–10723 (2017). Article CAS Google Scholar Chen, Z., Yang, Y., Yamaguchi, H., Hung, M.-C. & Kameoka, J. Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform. Biomicrofluidics 14, ...
Several other advanced methods have been developed to isolate exosomes, such as integrated microfluidic device (Liang et al.,2017b), nanoplasmon-enhanced scattering (nPES; Liang et al.,2017a), membrane-mediated exosome isolation (Zhang et al.,2017), and on-chip exosome isolation (Liu et al....
Extensive research on exosome potential in regenerative medicine is being carried out and researchers are exploring its use in the treatment of degenerative disorders such as Parkinson's disease. Doxakis (2010), Fragkouli and Doxakis (2014) and Kabaria et al. (2015) have reported mesenchymal ...
However, current isolation approaches are burdensome and suffer from low speed, yield and purity, limiting basic research and clinical applications. Here, we describe an efficient exosome detection method via the ultrafast-isolation system (EXODUS) that allows automated label-free purification of ...
Then, the medium was filtered through 0.22-µm-pore-size nitrocellulose membrane, the supernatant was added to 7.5 mL of total exosome isolation reagent and the samples were mixed and incubated overnight at 4 °C. Then, samples were centrifuged at 13,000 × g at 4 °C for 70 min. ...
can avoid the attack of immune cells by forming a hydration layer, therefore, engineered exo somes achieve a higher stability and longer circulation time.42 In addition, exosomes modified with magnetic compounds can be effectively applied for exosome detection, isolation and targeted drug delivery...
The internalization was measured as the total fluorescence intensity of VybrantDiO-sEVs, divided by the total number of cells by condition (Fig. 7f, g). Despite the lower levels of internalization of sEVs in the BBB-on-a-chip as compared to the static BBB model, when incubating it with ...
markers using magnetic beads. Magnetic beads are versatile tools for exosome isolation and downstream analysis [49]. Immunomagnetic isolation uses the antibody-labeled magnetic beads which can specifically capture exosomes and the magnetic field will separate the captured exosome from other substances [50...
Isolation and purification of exosomes The exosomes secreted by the cultured primary MSCs were isolated by use of the ExoQuick™ solution (System Biosciences; Palo Alto, CA) as per the standard method provided by supplier. The isolated exosome pellets were treated with bicinchoninic acid (BCA) ...