Here, we found that cholecystokinin (CCK)-labeled duodenal neuropod cells differentiate and transduce luminal stimuli from sweeteners and sugars to the vagus nerve using sweet taste receptors and sodium glucose transporters. The two stimulus types elicited distinct neural pathways: while sweetener ...
Then, we explored the underlying molecular changes of HS tissues under the drug-loaded MNs and 5-Fu injection treatment. The Venn diagram software was used to identify the commonly DEGs between drug-loaded MNs and 5-Fu injection modalities, and a total of 102 DEGs were commonly shared (Fig....
Serum and supernate of homogenized tissue were thawed, mixed with isotopically labeled internal standard solutions and then transferred to new tubes after vortex and centrifugation (29,700 g, 10 min, 4 ℃). The cryogenic vacuum centrifugal enrichment and evaporation system (CentriVap Labconco) was ...
The NanoString nCounter uses multiplexed nucleotide probes, labeled with a fluorophore, to generate counts for each gene, as previously described [21]. RNA (50 ng) was prepared as detailed above. RNA expression was quantified on the nCounter Digital AnalyzerTM, and raw and adjusted counts ...
(Bioss, Beijing, China) overnight at 4 °C. On the second day, slides were incubated with the HRP-labeled anti-rabbit IgG secondary antibody for 30 min at room temperature. Subsequently, the sections were stained with DAB according to the manufacturer’s instructions (ZSGB-BIO, Beijing, ...
The second set of slides were double-labeled with anti-GFAP (Glial Fibrillary Acidic Protein) and anti-MCP-1 (Monocyte Chemotactic Protein-1) to visualize astrocytes. GFAP is one of a family of intermediate filament proteins essential for the process of reactive astrogliosis, and MCP-1 is a ...
(RRID:CVCL_2959) in 96-well plates prepared 48–72 h before the experiment. Cells were incubated for 30 min to allow for attachment. Unattached cells were removed with PBS washes. Attachment of the fluorescently labeled monocytes was quantified using the SpectraMax i3x Multi-Mode Microplate...
Microarray analysis was performed with labeled cDNA hybridized to Agilent Human lncRNA Microarray V6 (4∗180K), a NimbleGen Hybridization System according to the manufacturer’s protocol. After hybridization and washing, the processed pictures were scanned with the Agilent Microarray Scanner. Identifica...
After infection (24 h, Vero E6, Pasteur; 16 h, VAT-1), cells were fixed with a final concentration of 4% formaldehyde, permeabilized with 0.2% Triton X-100 and 3% BSA in PBS (vol/vol) and stained for the SARS-CoV-2 N protein using the Alexa 488-labeled CR3009 antibody ...
6c). To confirm the observed effects of the inhibitor on the interactome we performed a western blot analysis of the biotin-labeled proteins after pull-down (Supplementary Fig. 6d). Also using this orthogonal technique, we see a general loss of labeled interactors upon treatment with the ...