Recombinant inbred lines from the diploid A- and B-genome populations were shotgun sequenced to 1× genome coverage with paired-end 100-bp reads on a HiSeq 2500 sequencer. The parents were sequenced at 20× genome coverage. Parental-homozygous SNPs were identified by alignments to the scaffolds...
Preparation of libraries for DNA sequencing for Illumina systems involves multiple steps. In a general workflow, purified DNA is fragmented, end-repaired, and A-tailed; adapters are ligated to the DNA fragments; libraries are ...
The organellar genomes were sequenced using a Sanger sequencer and assembled separately (Supplementary Methods; Supplementary Figs 4 and 5). Scaffolding using Illumina longer range mate-pair libraries and subsequent gap-filling using PacBio reads increased the N50 to 3.72 Mb. The assembly ...
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications. ...
Before you shutdown the DNA sequencer and close your pipette tip boxes, help your colleagues make the most of MacVector and give the gift ofMacVector Weekly Tips) for Christmas. We curate thesetipsfrom the most common support calls and emails that we receive. Please encourage any of your col...
Sequencer generated binary files (ABIF - Applied Biosystems Inc. Format files) were subsequently analyzed using the open-access tool Chromas Plite 2.6 (https://chromas.software.informer.com/2.6/). In case of a discrepancy between Sanger sequencing and NGS results, DNA was purified again from an...
The sequencing libraries were subsequently sequenced on an Illumina NextSeq 500 sequencer in paired-end mode. Sequencing results were demultiplexed using Illumina bcl2fastq (v.2.20), requiring a perfect match to indexing BC sequences. Low-quality reads or bases and Illumina adapter sequences were ...
Indeed, the development of, e.g., the MinION device (Oxford Nanopore Technologies), a portable nanopore real-time sequencer that requires just small DNA or RNA amounts, would enable the clear detection of signs of life [72]. Among the other potential biosignatures investigated, we have ...
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4). Additionally, four 20-kb mate-pair libraries were sequenced on a Roche 454 Titanium sequencer, amounting to 581 Mb of raw data (Supplementary Table 26). The resulting sequences were filtered for duplicates using custom Python scripts. The RH BACs were sequenced using a combination of ...