The combination of mycelial growth and dispersal of spores through soil are significant in the spread of genes in a particular population (Wellington et al. 1990). The major roles of streptomycetes in soil include the later stages of decomposition of recalcitrant polymers such as cellulose, lignin...
PHASTEST analyzes the proportion of phage proteins within a bacterial genomic region and scores the potential phages based on their length and inclusion of several phage-related proteins, such as “capsid,”“head,”“integrase,”“plate,”“tail,”“fiber,”“coat,”“transposase,”“portal,”...
Some characteristics of the lytic development of the temperate phage phi C31 in Streptomyces coelicolor A3(2) were studied using a thermoinducible lysogen. The physiological state of the host and the culture medium influenced the production of progeny virus after induction. The latent period lasted ...
Photosynthesis is the utilization of radiant energy for the synthesis of complex organic molecules. The phototrophic way of life implies the capture of electromagnetic energy (see section “Light Absorption and Excitation Transfer in Prokaryotes” in thi
A VIRULENT actinophage destructive to S. aureofaciens cultures and chlortetracycline (the trade mark of American Cyanamid Company for chlortetracycline is 'Aureomycin') fermentations had previously been reported 1 . Limited data regarding its host-range obtained at that time showed that it was a monov...
Despite growing interest in the above topics, much of what we know about phage biology is dependent on a small cadre of phages (e.g., the T-phages, lambda, SPP1). SPP1 was isolated in 1968 from soil in the Botanical Garden of Pavia, Italy [24] and has been the subject of numerous...
One unit of lipase activity is defined as the amount of LysB-His6 enzyme that increases the optical density OD400nm of 0.01/min under the assay conditions. Optimum temperature, pH, as well as the effect of metal ions and enzyme inhibitors on the lipase activity were also determined as ...
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As all three terminators either overlap with or lie close to sequences which interact with phage repressor proteins (conserved inverted repeats, CIRs) and these can potentially form stem-loop structures in RNA, the effect of CIRs on termination was also investigated. Termination at etb was ...
Adaptors and residual synthetic constructs were removed first mapping the reads on the genome sequence of the phage Phy174, and then using trim galore [47]. Microbiome profiling based on a database of taxon-specific marker genes was performed using MetaPhlAn2 [48]. The output of MetaPhlAn2 ...