The Dual-Luciferase Reporter Assay System (Yeasen, Shanghai, China) was utilized in order to evaluate the luciferase activity of the VEGFA promoter. The evaluation was carried out in accordance with the directions that were published. In this study, MKN-28 and HGC-27 cell lines were subjected...
Luciferase activity assessed by the Dual Luciferase Reporter Assay System.Shasha, ZhuXiaoshan, HuZhangbin, YuYuzhu, PengJingai, ZhuXuehua, LiuMengmeng, LiShuping, HanChun, Zhu
The cells were then used for the luciferase assay using the Dual-Luciferase Reporter Assay System (Promega). Relative luciferase activity was defined as the ratio of firefly luciferase activity to Renilla luciferase activity. Coimmunoprecipitation (Co-IP) and immunoblotting...
The Dual-Luciferase®Reporter Assay System (Promega, Fitchburg, WI, USA) was used to measure the different luciferase values, according to the manufacturer’s instructions. Transfections were done with 1 μg of pA10-luc (gift from W-J Lee) or pA10△Oct-luc construct (this work) and ...
Luciferase activities were detected by use of the Dual-Luciferase Reporter Assay System (Promega, E1910) according to the manufacturer’s instructions. Molecular docking simulation The 3D structure of the γ-linolenic acid was downloaded from PubChem, and then the structure was imported into ChemBio...
and the lysates were analyzed for luciferase activity using the Dual Luciferase Reporter assay (Promega) in a GloMax™ Microplate Luminometer (Promega). ... P Corporation 被引量: 0发表: 0年 Using the GloMax®-Multi Detection System for Measuring Signal from Multiplexed... and the lysates ...
2.11. Luciferase activity assay Luciferase activity was detected using the Dual-luciferase Reporter Assay System (Promega, USA). For relative luciferase activity assay, the value of Firefly-luciferase was normalized to the value of Renilla-luciferase. Briefly, 1 × 105 A549 cells/well were seeded ...
After 48 h of transfection, luciferase activities were measured by using a dual-luciferase reporter assay system. All experiments were repeated three times. 2.5. Subcutaneous Xenograft Tumor Model Female nude mice (18–20 g) aged 4–6 weeks old were purchased from Beijing Vital River ...
Cells were transfected with a firefly luciferase-containing reporter plasmid (4EBP3 promoter) and Renilla luciferase-containing plasmid as an internal control. Relative activity of firefly luciferase to Renilla luciferase was determined using the Dual-Glo luciferase assay system. Cells were seeded in 24...
Luciferase activity was quantified using reagents from the Dual Luciferase Reporter Assay System (Promega, E2920) according to the manufacturer’s instructions. Analysis of ChIP-seq data Trimmomatic-0.3870 was used for trimming with the following parameters: minimum length of 36 bp; mean Phred qual...